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Product Name
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DAB Enhancer
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Product type
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IHC reagents
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Description
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DAB Enhancer
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Tested applications
(see key)
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IHC-Fr, IHC-P
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Application notes
(see key)
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Recommended dilutions
i) Add one drop (50ul) of DAB enhancer in 1 ml of DAB incubation buffer.
ii) Add DAB chromogen and hydrogen peroxide to the above buffer.
iii) Incubate tissue sections with the incubation medium.
(Please note that DAB chromogen and hydrogen peroxide are not included in this kit, and must be purchased separately).
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Research areas
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Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents
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Relevance
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DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted amongst pathologists because of its superior performance as compared to amino ethylcarbazole (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. This is a stable liquid solution which increases the staining intensity of the tissue specimens stained with DAB several fold and hence increases the efficiency of detection systems.
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Form
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Liquid
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Storage instructions
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Store at room temperature.
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Notes
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To use as DAB signal enhancer in conjunction with peroxidase based immunostaining systems.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DAB Enhancer is on this datasheet. But please do contact us if you would like any reassurance! |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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