You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameDAB Enhancer
  • General notesTo use as DAB signal enhancer in conjunction with peroxidase based immunostaining systems.

    i) Add one drop (50?L) of DAB enhancer in 1mL of DAB incubation buffer.
    ii) Add DAB chromogen and Peroxidase Block (3% Hydrogen Peroxide) to the above buffer.
    iii) Incubate tissue sections with the incubation medium.

    • Properties

  • FormLiquid
  • Storage instructionsStore at room temperature.
  • Concentration information loading...
  • Reagent notes DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted amongst pathologists because of its superior performance as compared to amino ethylcarbazole (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. This is a stable liquid solution which increases the staining intensity of the tissue specimens stained with DAB several fold and hence increases the efficiency of detection systems.
  • Research Areas

    • References for DAB Enhancer (ab675)

    This product has been referenced in:
    • Fernandez S  et al. Nasal immunity to staphylococcal toxic shock is controlled by the nasopharynx-associated lymphoid tissue. Clin Vaccine Immunol 18:667-75 (2011). Read more (PubMed: 21325486) »

    See 1 Publication for this product

    Publishing research using ab675 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab675 Submit a Question for ab675

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

    10
    Votes: Highest First

    Thank you for your prompt response. Do you recommend any buffer to go with the heat mediated retrieval, citrate, Tris/EDTA? DAB detection system would suit, I see you have detection kits such as http://www.abcam.com/Mouse-and-Rabbit-Specific-HRPDAB-ABC-de...

    Read More

    Thank you for contacting us.
    The kit will work with the rabbit and mouse primary antibodies.
    You can try citrate buffer pH for a start and Tris/EDTA in case the staining does not work. Please check the following for protocol and troubleshooting ...

    Read More

    I am enquiring about Rhotekin 2 primary antibody for use with IHC-P. I see you have an antibody clone 2C2, can you provide any information on appropriate epitope retrieval methods and detection kits or secondary antibody that would be suitable for use wit...

    Read More

    Thank you for contacting us.
    Heat mediated antigen retrieval method with microwave or pressure cooker is recommended for this ab.
    The suitable detection system depends on the substrate you want to use. e.g. with DAB or AEC, we have following pro...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"