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DAB substrate kit (ab94665)

Q&A (7)

Overview

  • Product nameDAB substrate kitSee all DAB substrate kit kits ...
  • Product overview

    This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.

  • Notes

    Contains DAB in buffer. Reagent is acidic and can cause burns if skin contact occurs.

    Contains Hydrogen Peroxide in DAB substrate buffer. Avoid contact with skin and eyes.

  • Tested applicationsIHC-P more details

Properties

  • Relevance3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol.
    • Alternative names
        3 3' Diaminobenzidine

    Applications

    Our Abpromise guarantee covers the use of ab94665 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P

    DAB substrate kit images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAB substrate kit (ab94665)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAB substrate kit (ab94665)
      Human colon carcinoma stained with anti-p53 antibody using DAB Substrate Kit (ab94665).

    Protocols

    References for DAB substrate kit (ab94665)

    ab94665 has not yet been referenced specifically in any publications.

    Publishing research using ab94665 ? Please let us know so that we can cite the reference in this datasheet

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    Displaying 1 - 7 of 7 results for Abreviews and Q&A

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    Inquiry: Hi there, I was wondering whether you can help. I have to stain mouse tissue using an unconjugated primary antibody (Anti-Mouse/Rat MULT1, Host - Armenian Hamster IgG) and a biotinylated secondary one (Anti-Armenian Hamster IgG Biotin, Host - Goa...

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    Thank you for your inquiry.

    I can suggest to use a strepavidin that is coupled to an enzyme, for example: ab128985 (Streptavidin Peroxidase (Ready-To-Use)) and combine this with a suitable chromogen, for example: ab94665 (DAB substrate kit)
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    Hi,



    Step 4 of this protocol says to “rinse tissue, counterstain.”



    http://www.abcam.com/DAB-substrate-kit-ab94665.html



    Rinse tissue with what? Water? PBS? PBST? TBST? Some oth...

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    Hello!

    In-house we use PBST. Although we do not have any experimental data using other buffers I would imagine that whatever buffer you are using to rinse the slides would be suitable as well.

    Please do not hesitate to contact me with ...

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     I would like to inform you that I plan to use Peroxide/DAB the substrate and chromogen for horseradish peroxidase as detection system I appreciate you for your kind help                                                       with my best regards      ...

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    Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the choi...

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    Dear Based on datasheet I suppose both products (ab64238 and ab94665) are kind the same, differing just on volume. If not, could you please point out the differences and suggest which one should I buy for an IHC analysis (my secondary Ab is a anti-Rabbit-...

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    Thank you for contacting us. You are correct, these are the same product, just different sizes. The appropriate size will depend on how many slides you anticipate needing to stain. If you prepare the substrate/chromogen mixture as suggested in the protoco...

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    I need to develop double IHC analysis on formalin-fixed paraffin-embedded (FFPE) tissue sections of human tumors specimens, and after we have to do laser capture microdissection of positive cells.   I already have two primary antibodies directed against...

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    Thank you for contacting us.

    We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT is ...

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    Can you please send me a protocol for using ab97546 in ICC with HRP / DAB detection

    Thank you for contacting us. I would recommend the following protocol for your purposes:

    Fixation

    Fix the samples either in ice-cold methanol, acetone (1-10 min) or in 3-4% paraformaldehyde in PBS pH 7.4 for 15 min at room temperature....

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    Good morning, I'm currently using Abcam products to perform imunohistochemistry on paraffinon my research protocols and I have some doubts that I would likeAbcam tohelp me. I worked with Anti-Survivin antibody (ab469) as a primary antibody in a 1/500 dilu...

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    Thank you for contacting us. I'm glad to hear you have been getting good staining with ab469. In order to help you obtain more intense staining would you mind filling in the form which I have attached to this email. It will allow me to more fully underst...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"