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  • Product nameDAB substrate kit
    See all DAB substrate kit kits
  • Product overview

    This product is available in a two component form consisting of a liquid, refrigerator stable DAB Chromogen and DAB Substrate. Once the two components are combined, the reagent can be used for up to six hours, making it ideal for automated stainers.

  • Notes

    Contains DAB in buffer. Reagent is acidic and can cause burns if skin contact occurs.

    Contains Hydrogen Peroxide in DAB substrate buffer. Avoid contact with skin and eyes.

  • Tested applicationsIHC-Pmore details


  • Relevance3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. In the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol.
  • Alternative names
    • 3 3' Diaminobenzidine


Our Abpromise guarantee covers the use of ab94665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

DAB substrate kit images

  • Human colon carcinoma stained with anti-p53 antibody using DAB Substrate Kit (ab94665).


References for DAB substrate kit (ab94665)

This product has been referenced in:

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Abreview for ab94665 - DAB substrate kit

Excellent Good 4/5 (Ease of Use)
This kit is streamlined to use at ease. The results is fantastic.

Abcam user community

Verified customer

Submitted Aug 07 2015

Thank you for your inquiry.

I can suggest to use a strepavidin that is coupled to an enzyme, for example: ab128985 (Streptavidin Peroxidase (Ready-To-Use)) and combine this with a suitable chromogen, for example: ab94665 (DAB substrate kit) <...

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In-house we use PBST. Although we do not have any experimental data using other buffers I would imagine that whatever buffer you are using to rinse the slides would be suitable as well.

Please do not hesitate to contact me wi...

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Thank you for contacting us. You are correct, these are the same product, just different sizes. The appropriate size will depend on how many slides you anticipate needing to stain. If you prepare the substrate/chromogen mixture as suggested in the prot...

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Thank you for contacting us.

We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT ...

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Thank you for contacting us. I would recommend the following protocol for your purposes:


Fix the samples either in ice-cold methanol, acetone (1-10 min) or in 3-4% paraformaldehyde in PBS pH 7.4 for 15 min at room temperatu...

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Thank you for contacting us. I'm glad to hear you have been getting good staining with ab469. In order to help you obtain more intense staining would you mind filling in the form which I have attached to this email. It will allow me to more fully unde...

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