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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-DAB2 antibody
See all DAB2 products (5) ...
Description
Rabbit polyclonal to DAB2
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Zebrafish, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human DAB2.
(Peptide available as ab33440.)
Positive control
This antibody gave a positive signal in Human Testis Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - DAB2 antibody (ab33441)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 83, 58.8 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
DAB2 is a component of the CSF1 signal transduction pathway. DAB2 mRNA is expressed in normal ovarian epithelial cells but is down regulated or absent from ovarian carcinoma cell lines. The down regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2 like domains, alpha and beta).
Cellular localization
Cytoplasmic vesicle; clathrin coated vesicle; clathrin coated vesicle membrane. Cell membrane; coated pit; clathrin coated pit. Note: Colocalizes with large insert containing isoforms of MYO6 at clathrin coated pits/vesicles.
Alternative names
- DAB 2 antibody
- Differentially expressed protein 2 antibody
- Disabled (Drosophila) homolog 2 (mitogen responsive phosphoprotein) antibody
see all
Database links
- Entrez Gene: 1601 Human
- Entrez Gene: 404040 Zebrafish
- Omim: 601236 Human
- SwissProt: P98082 Human
- SwissProt: Q6P0K3 Zebrafish
- SwissProt: Q6PFL4 Zebrafish
- SwissProt: Q7SYH8 Zebrafish
- Unigene: 481980 Human
Anti-DAB2 antibody images:
Western blot - DAB2 antibody (ab33441)
Anti-DAB2 antibody (ab33441) at 2 µg/ml + Testis (Human) Tissue Lysate - adult normal tissue (ab30257) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 83, 58.8 kDa
Observed band size : 102 kDa (why is the actual band size different from the predicted?)
This antibody was raised against an immunogen that is predicted to cross react with both isoforms 1 and 2 of Human DAB2 . We believe that the band observed is isoform 1 due to its higher molecular weight of 102 kDa. While this differs to its predicted molecular weight of 83 kDa (Swiss-Prot data) this migration has been observed in the literature and may be due to modifications at potential phosphorylation sites. (Am J Pathol. 2006 Jul;169(1):258-67. PMID: 16816378).
References for Anti-DAB2 antibody (ab33441)
This product has been referenced in:
- Abrami Let al. Endocytosis of the anthrax toxin is mediated by clathrin, actin and unconventional adaptors. PLoS Pathog 6:e1000792 (2010). WB; Human.Read more (PubMed: 20221438) »
See 1 publication for this product
Publishing research using ab33441? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"