Anti-DAB2IP antibody (ab87811)
Key features and details
- Rabbit polyclonal to DAB2IP
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DAB2IP antibody
See all DAB2IP primary antibodies -
Description
Rabbit polyclonal to DAB2IP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human DAB2IP aa 850-950.
Database link: Q5VWQ8 -
Positive control
- WB: human HeLa, mouse TCKMK-1, Renca, 4T1 and CT26.WT cells IP: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab87811 was affinity purified using an epitope specific to DAB2IP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 132 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
DAB2IP is a Ras GTPase-activating protein (GAP) that acts as a tumor suppressor gene and is inactivated by methylation in prostate and breast cancers. It also interacts with MAP3K5 to disrupt the association of MAP3K5 with the inhibitory 14-3-3 complex. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 153090 Human
- Entrez Gene: 69601 Mouse
- Entrez Gene: 192126 Rat
- Omim: 609205 Human
- SwissProt: Q5VWQ8 Human
- SwissProt: Q3UHC7 Mouse
- SwissProt: Q6P730 Rat
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Alternative names
- AF9Q34 antibody
- AIP1 antibody
- ASK interacting protein antibody
see all
Images
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Panel A: Western blot of 0.04µg/ml ab87811 with Lane 1: 50µg, Lane 2: 15g, Lane 3: 5g, HeLa whole cell lysate.
Panel B: HeLa cells with Lane 1: ab87811, Lane2: ab87811 lot: A302-439A, Lane 3: ab87811 lot: A302-440A, Lane 3: Control IgG
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All lanes : Anti-DAB2IP antibody (ab87811) at 0.1 µg/ml
Lane 1 : TCKMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 2 : Renca (murine renal carcinoma cell line) whole cell lysate
Lane 3 : 4T1 (murine mammary carcinoma cell line) whole cell lysate
Lane 4 : CT26.WT (mouse colon carcinoma cell line) whole cell lysate
Predicted band size: 132 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab87811 has been referenced in 18 publications.
- Tong Z et al. DAB2IP predicts treatment response and prognosis of ESCC patients and modulates its radiosensitivity through enhancing IR-induced activation of the ASK1-JNK pathway. Cancer Cell Int 22:106 (2022). PubMed: 35248066
- Wang G et al. Loss of DAB2IP Contributes to Cell Proliferation and Cisplatin Resistance in Gastric Cancer. Onco Targets Ther 14:979-988 (2021). PubMed: 33603402
- Lin CJ et al. DAB2IP modulates primary cilia formation associated with renal tumorigenesis. Neoplasia 23:169-180 (2021). PubMed: 33341566
- Wu G et al. DAB2IP decreases cell growth and migration and increases sensitivity to chemotherapeutic drugs in colorectal cancer. Ann Transl Med 9:1317 (2021). PubMed: 34532454
- Yun EJ et al. Wnt/ß-catenin signaling pathway induces autophagy-mediated temozolomide-resistance in human glioblastoma. Cell Death Dis 11:771 (2020). PubMed: 32943609