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All lanes : Anti-DACH1 antibody [EPR2841Y] (ab77234) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 79 kDa
Observed band size : 97 kDa (why is the actual band size different from the predicted?)
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