![Western blot - DACH1 antibody [EPR2841Y] (ab77234) image](#ab77234.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-DACH1 antibody [EPR2841Y]
See all DACH1 products (4) ...
Description
Rabbit monoclonal [EPR2841Y] to DACH1
Tested applications
WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
A synthetic peptide corresponding to residues near the C terminus of human DACH1
Positive control
MCF-7 and HeLa cell lysates
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2841Y
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Western blot - DACH1 antibody [EPR2841Y] (ab77234)
![Western blot - DACH1 antibody [EPR2841Y] (ab77234) image](/ps/datasheet/Images/77/ab77234/ab77234.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Flow Cyt
Application notes
Flow Cyt: 1/120.
IP: 1/40.
WB: 1/500 - 1/1000.Predicted molecular weight: 79 kDa.
Is unsuitable for ICC/IF or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
Tissue specificity
Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
Sequence similarities
Belongs to the DACH/dachshund family.
Domain
The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UI36
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DACH antibody
- Dach1 antibody
- DACH1_HUMAN antibody
see all
Database links
- Entrez Gene: 1602 Human
- Entrez Gene: 13134 Mouse
- Omim: 603803 Human
- SwissProt: Q9UI36 Human
- SwissProt: Q9QYB2 Mouse
- Unigene: 129452 Human
- Unigene: 320593 Mouse
Anti-DACH1 antibody [EPR2841Y] images:
Western blot - DACH1 antibody [EPR2841Y] (ab77234)
All lanes : Anti-DACH1 antibody [EPR2841Y] (ab77234) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 79 kDa
Observed band size : 97 kDa (why is the actual band size different from the predicted?)
References for Anti-DACH1 antibody [EPR2841Y] (ab77234)
ab77234 has not yet been referenced specifically in any publications.
Publishing research using ab77234? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"