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Western blot - DACH1 antibody (ab31588)
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Product Name
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DACH1 antibody
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See all DACH1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DACH1
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Immunogen
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Synthetic peptide derived from within residues 550 - 650 of Human DACH1.
(Peptide available as ab31587.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Chk
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent dilution (PMID 14525983).
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate ;
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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DACH1 is similar to the Drosophila melanogaster dachshund gene, which encodes a nuclear factor essential for determining cell fates in the eye, leg, and nervous system of the fly. It is a member of the Ski gene family and is thought to be involved in eye development. Four alternatively spliced transcripts encoding different isoforms have been described for this gene. (from Entrez gene). DACH1 has also been found to inhibit oncogene-mediated breast tumor growth via downregulation of cyclin D1. Expression of DACH1 has been found to inversely correlate with metastasis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 0.20 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DACH1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DACH1 antibody images, references, products related to ab31588 and other tools.
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DACH1 antibody images:
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References for DACH1 antibody (ab31588)
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This product has been used in:
Wu K et al. DACH1 inhibits transforming growth factor-beta signaling through binding Smad4. J Biol Chem 278:51673-84 (2003). WB, IHC-P; Human. PubMed: 14525983
If you publish research using ab31588 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to DACH1
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DACH1 antibody - more information
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Customer reviews (feedback) regarding DACH1 antibody |
Customer FAQs regarding DACH1 antibody |
Protocols for DACH1 antibody |
Price and availability of products related to DACH1 antibody |
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DACH1 antibody - related products:
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DACH1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DACH1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
Western blot - DACH1 antibody (ab31588)