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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-DACH1 antibody
See all DACH1 products (4) ...
Description
Rabbit polyclonal to DACH1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken
Immunogen
Synthetic peptide derived from within residues 550 - 650 of Human DACH1.
(Peptide available as ab31587.)
Positive control
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate; MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate ;
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
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Western blot - DACH1 antibody (ab31588)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
IHC-P: Use at an assay dependent dilution. (PubMed: 19605405)
Target
Function
Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (By similarity). Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
Tissue specificity
Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
Sequence similarities
Belongs to the DACH/dachshund family.
Domain
The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UI36
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DACH antibody
- Dach1 antibody
- DACH1_HUMAN antibody
see all
Database links
- Entrez Gene: 373935 Chicken
- Entrez Gene: 1602 Human
- Entrez Gene: 13134 Mouse
- Omim: 603803 Human
- SwissProt: Q9UI36 Human
- SwissProt: Q9QYB2 Mouse
- Unigene: 129452 Human
- Unigene: 320593 Mouse
Anti-DACH1 antibody images:
Western blot - DACH1 antibody (ab31588)
All lanes : Anti-DACH1 antibody (ab31588) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 79 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
References for Anti-DACH1 antibody (ab31588)
This product has been referenced in:
- Zhou Jet al. Attenuation of Forkhead signaling by the retinal determination factor DACH1. Proc Natl Acad Sci U S A 107:6864-9 (2010). WB; Human.Read more (PubMed: 20351289) »
- Zhou Jet al. Transcription Elongation Regulator 1 Is a Co-integrator of the Cell Fate Determination Factor Dachshund Homolog 1. J Biol Chem 285:40342-50 (2010).Read more (PubMed: 20956529) »
See all 3 publications for this product
Publishing research using ab31588? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"