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Anti-DACH2 antibody (ab77688)

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Overview

Product name

Anti-DACH2 antibody
See all DACH2 products (2) ...

Description

Goat polyclonal to DACH2

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat, Horse, Dog

Immunogen

Synthetic peptide: TRKQAVNSSRPGR by a Cysteine residue linker, corresponding to internal sequence amino acids 169-181 of Human DACH2

TRKQAVNSSR PGR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

NIH3T3 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other

  • Western blot - DACH2 antibody (ab77688)Western blot - DACH2 antibody (ab77688) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Peptide ELISA: Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa according to mouse NP_291083.1 and 65 kDa according to human NP_444511.1).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

DACH2 is a transcription factor that is involved in regulation of organogenesis. It seems to be a regulator for SIX1 and SIX6 and may act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. It probably binds to DNA via its DACH box-N domain.

Cellular localization

Nuclear

Alternative names

  • DACH 2 antibody
  • Dachshund 2 antibody
  • Dachshund homolog 2 (Drosophila) antibody
  • Dachshund homolog 2 antibody
  • Dachshund2 antibody
  • FLJ31391 antibody
  • HGNC:16814 antibody
  • MGC138545 antibody
see all

Database links

see all

Anti-DACH2 antibody images:

  Western blot - DACH2 antibody (ab77688)

Western blot - DACH2 antibody (ab77688)

Anti-DACH2 antibody (ab77688) at 0.03 µg/ml + NIH3T3 cell lysate (in RIPA buffer) at 35 µg
developed using the ECL technique

Predicted band size : 69 kDa
Observed band size : 69 kDa


Primary incubation was 1 hour.

References for Anti-DACH2 antibody (ab77688)

ab77688 has not yet been referenced specifically in any publications.

Publishing research using ab77688? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"