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I am thinking of purchasing DAD1 antibody to use against Xenopus Laevis. The primary accession number is P46967, and the fasta format follows. >sp|P46967|DAD1_XENLA Defender against cell death 1 (DAD-1) - Xenopus laevis (African clawed frog). MSVSVFSVVSRFLDEYVSSTPQRLKLLDAYLLYILLTGALQFLYCLLVGTFPFNSFLSGFISSVGSFILAVCLRIQINPQNKSDFQGISPERAFADFLFANTILHLVVVNFIG I was wondering if you could give me an idea of the chance that your anti body will detect this version of the protein? Thank you |
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ANSWER: |
Thank you for your enquiry. I contacted the antibody originator regarding ab23836. Unfortunately the amino acid sequence of the immunogen is proprietary. The only information I have is that the sequence was selected from the last half of the protein, but not the very C-terminus. The amino acid sequence of human DAD1 is in the public domain (GenBank accession no. AAH09798). However, without access to the immunogen sequence I am not in a position to comment on the homology between the relevant sequence in human versus xenopus. I am sorry that I could not assist further. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Predicted band size : 12 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.
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