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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-DAD1 antibody
See all DAD1 products (2) ...
Description
Rabbit polyclonal to DAD1
Tested applications
WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a 14 amino acid sequence within the C terminal region of DAD 1 (Human).
Positive control
HepG2 cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Purified by affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2.0 µg/ml.Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).
ICC: Use at an assay dependent dilution.
Target
Function
Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
Sequence similarities
Belongs to the DAD/OST2 family.
Cellular localization
Membrane.
Target information above from: UniProt accessionP61803
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAD 1 antibody
- DAD-1 antibody
- dad1 antibody
see all
Database links
- Entrez Gene: 1603 Human
- Entrez Gene: 13135 Mouse
- Omim: 600243 Human
- SwissProt: P61803 Human
- SwissProt: Q6FGA3 Human
- SwissProt: P61804 Mouse
- Unigene: 82890 Human
- Unigene: 319038 Mouse
Anti-DAD1 antibody images:
Western blot - DAD1 antibody (ab23836)
Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml
Lane 1 : HepG2 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Predicted band size : 12 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry - DAD1 antibody (ab23836)
ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.
References for Anti-DAD1 antibody (ab23836)
This product has been referenced in:
- Murooka TTet al. CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation. Biochem Biophys Res Commun 387:381-6 (2009). WB; Human.Read more (PubMed: 19607806) »
See 1 publication for this product
Publishing research using ab23836? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"