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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab81984? Please let us know so that we can cite the reference in this datasheet
ab81984 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - DAGLA antibody (ab81984)
Anti-DAGLA antibody (ab81984) at 0.3 µg/ml + human liver lysate (in RIPA buffer) at 35 µg
developed using the ECL technique
Predicted band size : 115 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Some minor background is detected and is blocked by the immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAGLA antibody (ab81984)
Immunohistochemistical detection of DAGLA using ab81984 on formaldehyde fixed rat cerebellum tissue sections (cerebellum). Antigen retrieval step: heat mediated in citric acid pH6. 1%BSA as blocking agent for 10 mins@ rt°C. Primary antibody dilution 1/1000 for 2 hours. Secondary antibody: anti-sheep IgG conjugated to biotin (1/200). The pattern of immunolabelling is very similar to that obtained in mouse. However, the cell bodies of some Purkinje cells are also positive, this appears to be punctate and evenly distributed.
Carl Hobbs, King`s College London, United Kingdom
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAGLA antibody (ab81984)
Immunohistochemistical detection of DAGLA with ab81984 on formaldehyde fixed mouse cerebellum sections. Antigen retrieval step: heat mediated in citric acid pH6. 1% BSA used as blocking agent for 10 mins @ rt°C. Primary antibody ab81984 incubated at 1/500 for 2 hours. Secondary antibody: anti sheep IgG conjugated to biotin (1/200). The upper image shows DAGL immunostaining with ab81984 on wildtype mouse cerebellum, the lower image shows an absence of DAGL alpha immunostaining on knockout mouse mouse cerebellum. These results are consistent with published data with other anti-DAGL alpha antibodies (http://jcb.rupress.org/cgi/content/full/163/3/463)
Carl Hobbs, King`s College London, United Kingdom
DAGLA antibody for IHC-P in Mouse (81984)
DAGLA antibody for IHC-P in Rat (81984)
DAGLA antibody for IHC-P in Monkey (81984)
Anti-DAGLA antibody for Western blot in Mouse (81984)
DAGLA antibody for WB in Mouse (81984)
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