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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Lipases
Overview
Product name
Anti-DAGLA antibody
See all DAGLA products (2) ...
Description
Goat polyclonal to DAGLA
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Monkey
Immunogen
Synthetic peptide: C-PAKQDELVISAR, corresponding to C terminal amino acids 1031-1042 of Human DAGLA.
C-PAKQDELV ISAR
Positive control
Human liver lysates (WB). Mouse cerebellum (IHC)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Lipid metabolism
Neuroscience >> Neurology process >> Neurogenesis
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Lipases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.3 - 1 µg/ml.Predicted molecular weight: 115 kDa.
ELISA: Use at an assay dependent dilution.
IHC-P: 1/500 - 1/5000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
Tissue specificity
Highly expressed in brain and pancreas.
Involvement in disease
Defects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20) [MIM:608687]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult-onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. Note=A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA.
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ9Y4D2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C11orf11 antibody
- Chromosome 11 open reading frame 11 antibody
- DAGL(ALPHA) antibody
see all
Database links
- Entrez Gene: 747 Human
- Entrez Gene: 269060 Mouse
- Entrez Gene: 309207 Rat
- SwissProt: Q9Y4D2 Human
- SwissProt: Q6WQJ1 Mouse
- SwissProt: Q5YLM1 Rat
- Unigene: 241564 Human
- Unigene: 329718 Mouse
see all
Anti-DAGLA antibody images:
Western blot - DAGLA antibody (ab81984)
Anti-DAGLA antibody (ab81984) at 0.3 µg/ml + human liver lysate (in RIPA buffer) at 35 µg
developed using the ECL technique
Predicted band size : 115 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Some minor background is detected and is blocked by the immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAGLA antibody (ab81984)
Immunohistochemistical detection of DAGLA using ab81984 on formaldehyde fixed rat cerebellum tissue sections (cerebellum). Antigen retrieval step: heat mediated in citric acid pH6. 1%BSA as blocking agent for 10 mins@ rt°C. Primary antibody dilution 1/1000 for 2 hours. Secondary antibody: anti-sheep IgG conjugated to biotin (1/200). The pattern of immunolabelling is very similar to that obtained in mouse. However, the cell bodies of some Purkinje cells are also positive, this appears to be punctate and evenly distributed.
Carl Hobbs, King`s College London, United Kingdom
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAGLA antibody (ab81984)
Immunohistochemistical detection of DAGLA with ab81984 on formaldehyde fixed mouse cerebellum sections. Antigen retrieval step: heat mediated in citric acid pH6. 1% BSA used as blocking agent for 10 mins @ rt°C. Primary antibody ab81984 incubated at 1/500 for 2 hours. Secondary antibody: anti sheep IgG conjugated to biotin (1/200). The upper image shows DAGL immunostaining with ab81984 on wildtype mouse cerebellum, the lower image shows an absence of DAGL alpha immunostaining on knockout mouse mouse cerebellum. These results are consistent with published data with other anti-DAGL alpha antibodies (http://jcb.rupress.org/cgi/content/full/163/3/463)
Carl Hobbs, King`s College London, United Kingdom
References for Anti-DAGLA antibody (ab81984)
ab81984 has not yet been referenced specifically in any publications.
Publishing research using ab81984? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"