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Western blot - DAGLA antibody (ab81984)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAGLA antibody (ab81984)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAGLA antibody (ab81984)
(enlarge)
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Product Name
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DAGLA antibody
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Product type
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Primary antibodies
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Description
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Goat polyclonal to DAGLA
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Immunogen
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Synthetic peptide: C-PAKQDELVISAR, corresponding to C terminal amino acids 1031-1042 of Human DAGLA.
BLAST 'C-PAKQDELVISAR' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Mk
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: 1/500 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 115 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver lysates (WB). Mouse cerebellum (IHC)
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Cellular localization
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Cell membrane; Multi pass membrane protein.
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Research areas
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Signal Transduction >> Metabolism >> Lipid metabolism
Neuroscience >> Neurology process >> Neurogenesis
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Lipases
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Relevance
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Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DAGLA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DAGLA antibody images, references, products related to ab81984 and other tools.
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DAGLA antibody images:
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 Western blot - DAGLA antibody (ab81984)
DAGLA antibody (ab81984) at 0.3 µg/ml + human liver lysate (in RIPA buffer) at 35 µg
developed using the ECL technique
Predicted band size : 115 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Some minor background is detected and is blocked by the immunizing peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAGLA antibody (ab81984)
Immunohistochemistical detection of DAGLA using ab81984 on formaldehyde fixed rat cerebellum tissue sections (cerebellum). Antigen retrieval step: heat mediated in citric acid pH6. 1%BSA as blocking agent for 10 mins@ rt°C. Primary antibody dilution 1/1000 for 2 hours. Secondary antibody: anti-sheep IgG conjugated to biotin (1/200). The pattern of immunolabelling is very similar to that obtained in mouse. However, the cell bodies of some Purkinje cells are also positive, this appears to be punctate and evenly distributed. Carl Hobbs, King`s College London, United Kingdom
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAGLA antibody (ab81984)
Immunohistochemistical detection of DAGLA with ab81984 on formaldehyde fixed mouse cerebellum sections. Antigen retrieval step: heat mediated in citric acid pH6. 1% BSA used as blocking agent for 10 mins @ rt°C. Primary antibody ab81984 incubated at 1/500 for 2 hours. Secondary antibody: anti sheep IgG conjugated to biotin (1/200). The upper image shows DAGL immunostaining with ab81984 on wildtype mouse cerebellum, the lower image shows an absence of DAGL alpha immunostaining on knockout mouse mouse cerebellum. These results are consistent with published data with other anti-DAGL alpha antibodies (http://jcb.rupress.org/cgi/content/full/163/3/463) Carl Hobbs, King`s College London, United Kingdom
See Abreview
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Search PubMed (MEDLINE) for references to DAGLA
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DAGLA antibody - more information
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Customer reviews (feedback) regarding DAGLA antibody |
Customer FAQs regarding DAGLA antibody |
Protocols for DAGLA antibody |
Price and availability of products related to DAGLA antibody |
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DAGLA antibody - related products:
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DAGLA antibody - other tools:
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Contact Abcam with a Technical Enquiry about DAGLA antibody |
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