Overview
- Product nameAnti-DAGLB antibodySee all DAGLB primary antibodies ...
- DescriptionRabbit polyclonal to DAGLB
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog - Immunogen
Synthetic peptide corresponding to a region within internal amino acids 216-265 (STAELFSTYF SDTDLVPSDI AAGLALLHQQ QDNIRNNQEP AQVVCHAPGS) of Human DAGLB (NP_631918).
- Positive control293T cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab84733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 74 kDa.Can be blocked with DAGLB peptide (ab104607). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- FunctionCatalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.
- Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
- Cellular localizationCell membrane.
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Database links
- Entrez Gene: 221955 Human
- Entrez Gene: 231871 Mouse
- Entrez Gene: 304289 Rat
- SwissProt: Q8NCG7 Human
- SwissProt: Q91WC9 Mouse
- SwissProt: P0C1S9 Rat
- Unigene: 487498 Human
- Unigene: 469963 Mouse
- Unigene: 44074 Rat
see all
Target information above from: UniProt accession
Q8NCG7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Daglb antibodyDGL beta antibodyDGL-beta antibody
- DGLB_HUMAN antibodyDiacylglycerol lipase beta antibodyKCCR13L antibodySn1 specific diacylglycerol lipase beta antibodySn1-specific diacylglycerol lipase beta antibody
see all
Anti-DAGLB antibody images
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Western blot - DAGLB antibody (ab84733)Anti-DAGLB antibody (ab84733) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 74 kDa
Observed band size : 84 kDa (why is the actual band size different from the predicted?)
Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.
References for Anti-DAGLB antibody (ab84733)
ab84733 has not yet been referenced specifically in any publications.
