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All lanes : Anti-DAK antibody (ab66006) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from A549 cells
Lane 3 : Extracts from A549 cells, plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 59 kDa
Observed band size : 59 kDa
Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.
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