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Anti-DAK antibody (ab66006)

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Overview

Product name

Anti-DAK antibody
See all DAK products (5) ...

Description

Rabbit polyclonal to DAK

Specificity

This antibody detects endogenous levels of total DAK protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

A synthesized peptide derived from the internal region of human DAK.

Positive control

Extracts from K562 cells and A549 cells

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Metabolism >> Energy Metabolism

  • Western blot - DAK antibody (ab66006)Western blot - DAK antibody (ab66006) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects bands of approximately 59 and 26 kDa (predicted molecular weight: 59 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Catalyzes both the phosphorylation of dihydroxyacetone and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.

Sequence similarities

Belongs to the dihydroxyacetone kinase (DAK) family.
Contains 1 DhaK domain.
Contains 1 DhaL domain.

Target information above from: UniProt accessionQ3LXA3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ATP dependent dihydroxyacetone kinase antibody
  • Bifunctional ATP dependent dihydroxyacetone kinase/FAD AMP lyase (cyclizing) antibody
  • DAK antibody
  • DHA kinase antibody
  • Dha kinase/FMN cyclase antibody
  • DHAK_HUMAN antibody
  • Dihydroxyacetone kinase antibody
  • Dihydroxyacetone kinase 2 antibody
  • Dihydroxyacetone kinase 2 homolog (S. cerevisiae) antibody
  • Dihydroxyacetone kinase 2 homolog (yeast) antibody
  • FAD AMP lyase (cyclic FMN forming) antibody
  • FAD AMP lyase (cyclizing) antibody
  • FAD-AMP lyase (cyclic FMN forming) antibody
  • FAD-AMP lyase (cyclizing) antibody
  • FMN cyclase antibody
  • Glycerone kinase antibody
see all

Database links

see all

Anti-DAK antibody images:

  Western blot - DAK antibody (ab66006)

Western blot - DAK antibody (ab66006)

All lanes : Anti-DAK antibody (ab66006) at 1/500 dilution

Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from A549 cells
Lane 3 : Extracts from A549 cells, plus 5µg immunizing peptide

Lysates/proteins at 5 µg per lane.


Predicted band size : 59 kDa
Observed band size : 59 kDa
Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DAK antibody (ab66006)

ab66006 has not yet been referenced specifically in any publications.

Publishing research using ab66006? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"