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Western blot - DAK antibody (ab66006)
(enlarge)
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Product Name
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DAK antibody
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See all DAK antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DAK
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Immunogen
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A synthesized peptide derived from the internal region of human DAK.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody detects endogenous levels of total DAK protein.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects bands of approximately 59 and 26 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from K562 cells and A549 cells
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Research areas
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Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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DAK is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DAK antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DAK antibody images, references, products related to ab66006 and other tools.
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DAK antibody images:
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 Western blot - DAK antibody (ab66006)
All lanes : DAK antibody (ab66006) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from A549 cells
Lane 3 : Extracts from A549 cells, plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 59 kDa
Observed band size : 59 kDa
Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to DAK
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DAK antibody - more information
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Customer reviews (feedback) regarding DAK antibody |
Customer FAQs regarding DAK antibody |
Protocols for DAK antibody |
Price and availability of products related to DAK antibody |
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DAK antibody - related products:
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DAK antibody - other tools:
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Contact Abcam with a Technical Enquiry about DAK antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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