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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Overview
Product name
Anti-DAP Kinase 1 antibody
See all DAP Kinase 1 products (4) ...
Description
Rabbit polyclonal to DAP Kinase 1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Xenopus laevis
Immunogen
Synthetic peptide: TVFRQENVDDYY-C conjugated to KLH, corresponding to N terminal amino acids 2-13 of Human DAP Kinase 1
TVFRQENVDD YY-C
Positive control
Extracts of human Hela cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose and further purified by specific absorption on the corresponding ZIP-kinase peptide, to eliminate any possible cross reaction with the homologous ZIP-kinase protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
Application notes
IP: Use at 2.5 - 5 µg.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 10 ANK repeats.
Contains 1 death domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome.
Cellular localization
Cytoplasm. Colocalizes with the actin filament system.
Target information above from: UniProt accessionP53355
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAK1 antibody
- DAP K1 antibody
- DAP kinase 1 antibody
see all
Database links
- Entrez Gene: 1612 Human
- Entrez Gene: 69635 Mouse
- Entrez Gene: 446562 Xenopus laevis
- Omim: 600831 Human
- SwissProt: P53355 Human
- SwissProt: Q1W5W0 Human
- SwissProt: Q5VTK1 Human
- SwissProt: Q3UKP8 Mouse
see all
References for Anti-DAP Kinase 1 antibody (ab67286)
ab67286 has not yet been referenced specifically in any publications.
Publishing research using ab67286? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"