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Product Name
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DAP Kinase 1 antibody
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See all DAP Kinase 1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DAP Kinase 1
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Immunogen
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Synthetic peptide: TVFRQENVDDYY-C conjugated to KLH, corresponding to N terminal amino acids 2-13 of Human DAP Kinase 1
BLAST 'TVFRQENVDDYY-C' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Xl
(Due to sequence homology)
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2.5 - 5 µg.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts of human Hela cells.
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Cellular localization
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Cytoplasmic : Colocalizes with the actin filament system.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
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Relevance
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DAPK1 (Death-associated protein kinase 1) is a calmodulin dependent serine/threonine serine kinase which functions as a positive mediator of gamma-interferon induced programmed cell death.
DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy.
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DAP Kinase 1 antibody (ab67286) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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The antibody is affinity-purified using the immunizing peptide immobilized on agarose and further purified by specific absorption on the corresponding ZIP-kinase peptide, to eliminate any possible cross reaction with the homologous ZIP-kinase protein.
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Form
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Liquid
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Concentration
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Batch dependent within range: 1.00 - 1.20 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DAP Kinase 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to DAP Kinase 1
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DAP Kinase 1 antibody - more information
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Customer reviews (feedback) regarding DAP Kinase 1 antibody |
Customer FAQs regarding DAP Kinase 1 antibody |
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