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Overview

  • Product nameDAP Kinase 1 protein (Active)See all DAP Kinase 1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-363 of Human DAP Kinase 1, expressed by baculovirus in Sf9 insect cells using an N-terminal enzyme tag.
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purification notesThe purity was determined to be >80% by densitometry.
  • Biological activitySpecific activity: 49 nmol/min/mg.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab63191 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
    SDS-PAGE
  • Application notesKinase activity assay: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DAK1DAP K1DAP kinase 1
        DAPKDAPK 1DAPK1DAPK1_HUMANDeath Associated Protein Kinase 1Death-associated protein kinase 1DKFZp781I035
      see all
  • FunctionCalcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 10 ANK repeats.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm. Colocalizes with the actin filament system.

    • Target information above from: UniProt accession P53355 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    DAP Kinase 1 protein (Active) images

    • Functional Studies - DAP Kinase 1 protein (Active) (ab63191)
      Functional Studies - DAP Kinase 1 protein (Active) (ab63191)
      Sample Kinase Activity Plot.
    • SDS-PAGE - DAP Kinase 1 protein (Active) (ab63191)
      SDS-PAGE - DAP Kinase 1 protein (Active) (ab63191)
      ab63191 on SDS-PAGE. Molecular Weight: ~71 kDa.

    References for DAP Kinase 1 protein (Active) (ab63191)

    ab63191 has not yet been referenced specifically in any publications.

    Publishing research using ab63191 ? Please let us know so that we can cite the reference in this datasheet

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