![Western blot - DAP Kinase 2 antibody [EP1633Y] (ab51601) image](#ab51601 wb.bmp)
![Immunohistochemistry (Paraffin-embedded sections) - DAP Kinase 2 antibody [EP1633Y] (ab51601) image](#ab51601_2.JPG)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-DAP Kinase 2 antibody [EP1633Y]
See all DAP Kinase 2 products (12) ...
Description
Rabbit monoclonal [EP1633Y] to DAP Kinase 2
Specificity
ab51601 recognizes DAP Kinase 2.
Tested applications
WB, IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues on the N terminal of human DAP Kinase 2.
Positive control
WB: Rat spleen lysate IHC-P: Human liver carcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1633Y
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P: 1/50 - 1/100.
Flow Cyt
Flow Cyt: 1/15Flow Cyt: 1/15
Application notes
Is unsuitable for ,ICC/IF or IP.
Target
Function
Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
Tissue specificity
Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UIK4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAK 2 antibody
- DAK2 antibody
- DAP kinase 2 antibody
see all
Database links
- Entrez Gene: 23604 Human
- Entrez Gene: 13143 Mouse
- Entrez Gene: 300799 Rat
- Omim: 604727 Human
- SwissProt: Q9UIK4 Human
- SwissProt: Q8VDF3 Mouse
- Unigene: 237886 Human
- Unigene: 304472 Mouse
see all
Anti-DAP Kinase 2 antibody [EP1633Y] images:
Western blot - DAP Kinase 2 antibody [EP1633Y] (ab51601)
![Western blot - DAP Kinase 2 antibody [EP1633Y] (ab51601)](/ps/datasheet/Images/51/ab51601/ab51601 wb.bmp)
Anti-DAP Kinase 2 antibody [EP1633Y] (ab51601) at 1/2000 dilution + Rat spleen lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
Immunohistochemistry (Paraffin-embedded sections) - DAP Kinase 2 antibody [EP1633Y] (ab51601)
![Immunohistochemistry (Paraffin-embedded sections) - DAP Kinase 2 antibody [EP1633Y] (ab51601)](/ps/datasheet/Images/51/ab51601/ab51601_2.JPG)
Immunohistochemical staining of paraffin embedded human liver carcinoma using ab51601 (1/50). Secondary stained using Goat anti-rabbit HRP (1/2000).
References for Anti-DAP Kinase 2 antibody [EP1633Y] (ab51601)
This product has been referenced in:
- Shoval Yet al. New Modularity of DAP-Kinases: Alternative Splicing of the DRP-1 Gene Produces a ZIPk-Like Isoform. PLoS One 6:e17344 (2011). WB; Human.Read more (PubMed: 21408167) »
See 1 publication for this product
Publishing research using ab51601? Please let us know so that we can cite the reference in this datasheet
![DAP Kinase 2 antibody [EP1633Y] for WB in Human (51601)](/ps/Reviews/Images/ab11628_61647.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"