Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-DAP Kinase 2 antibody
See all DAP Kinase 2 products (12) ...
Description
Rabbit polyclonal to DAP Kinase 2
Specificity
Does not react with DAPK1.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Peptide (ARRKALHPRRRSSTS) corresponding to aminoacids 356 to 370 of human DAPK2 (1,2). The sequence ofthis antigenic peptide is identical to the correspondingamino acids of mouse origin (1,2)
ARRKALHPRR RSSTS
Positive control
A431 whole cell lysate. An approximately42 kDa band can be detected.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types ofcells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family ofserine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues (1).
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml
WB: Use at an assay dependent concentration.
Target
Function
Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
Tissue specificity
Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UIK4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAK2 antibody
- DAP kinase 2 antibody
- DAP Kinase Related Protein 1 antibody
see all
Database links
- Entrez Gene: 23604 Human
- Entrez Gene: 13143 Mouse
- Entrez Gene: 300799 Rat
- Omim: 604727 Human
- SwissProt: Q9UIK4 Human
- SwissProt: Q8VDF3 Mouse
- Unigene: 237886 Human
- Unigene: 304472 Mouse
see all
Anti-DAP Kinase 2 antibody images:
Western blot - DAP Kinase 2 antibody (ab8404)
All lanes : Anti-DAP Kinase 2 antibody (ab8404) at 1 µg/ml
Lane 1 : A431 whole cell lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat kidney lysate
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAP Kinase 2 antibody (ab8404)
ab8404 at 2µg/ml staining DAP kinase 2 in mouse spleen cells by IHC
References for Anti-DAP Kinase 2 antibody (ab8404)
ab8404 has not yet been referenced specifically in any publications.
Publishing research using ab8404? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"