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DAP Kinase 2 protein (Active) (ab89742)

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Overview

Product name

DAP Kinase 2 protein (Active)
See all DAP Kinase 2 products (3) ...

Protein description

Recombinant full length Human DAP Kinase 2 with an N-terminal terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system; amino acids 1-370, 67kDa. UniProt = Q9UIK4.

Properties

Purification notes

Purity was determined to be >80% by densitometry.

Biological activity

The Specific activity of ab89742 was determined to be 136 nmol/min/mg.

Form

Liquid

Storage instructions

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

Concentration

Concentration information loading...

  • SDS-PAGE - DAP Kinase 2 protein (Active) (ab89742)SDS-PAGE - DAP Kinase 2 protein (Active) (ab89742) image (enlarge)

  • Functional Studies - DAP Kinase 2 protein (Active) (ab89742)Functional Studies - DAP Kinase 2 protein (Active) (ab89742) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab89742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.

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    Functional Studies

     FuncS: Use at an assay d...Read more →

    FuncS: Use at an assay dependent dilution.

Protein info

Function

Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.

Tissue specificity

Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionQ9UIK4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

DAP Kinase 2 protein (Active) images:

  SDS-PAGE - DAP Kinase 2 protein (Active) (ab89742)

SDS-PAGE - DAP Kinase 2 protein (Active) (ab89742)

SDS-PAGE showing ab89742 at approximately 67kDa.

  Functional Studies - DAP Kinase 2 protein (Active) (ab89742)

Functional Studies - DAP Kinase 2 protein (Active) (ab89742)

Kinase Assay demonstrating specific activity of ab89742.

References for DAP Kinase 2 protein (Active) (ab89742)

ab89742 has not yet been referenced specifically in any publications.

Publishing research using ab89742? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"