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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
DAP Kinase 2 protein (Active)
See all DAP Kinase 2 products (3) ...
Protein description
Recombinant full length Human DAP Kinase 2 with an N-terminal terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system; amino acids 1-370, 67kDa. UniProt = Q9UIK4.
Properties
Purification notes
Purity was determined to be >80% by densitometry.
Biological activity
The Specific activity of ab89742 was determined to be 136 nmol/min/mg.
Form
Liquid
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
Concentration
Concentration information loading...
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab89742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.
FuncS: Use at an assay dependent dilution.
Protein info
Function
Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
Tissue specificity
Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UIK4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
DAP Kinase 2 protein (Active) images:
Functional Studies - DAP Kinase 2 protein (Active) (ab89742)
Kinase Assay demonstrating specific activity of ab89742.
References for DAP Kinase 2 protein (Active) (ab89742)
ab89742 has not yet been referenced specifically in any publications.
Publishing research using ab89742? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"