![Western blot - DAP1 antibody [E59] (ab32056) image](#ab32056-1.JPG)
![Immunofluorescence - DAP1 antibody [E59] (ab32056) image](#ab32056-2.JPG)
![Immunohistochemistry (Paraffin-embedded sections) - DAP1 antibody [E59] (ab32056) image](#ab32056-3.JPG)
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Product name
Anti-DAP1 antibody [E59]
See all DAP1 products (3) ...
Description
Rabbit monoclonal [E59] to DAP1
Tested applications
WB, IHC-P, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide within the last 25 amino acids at the C-terminus of human DAP1 (UniProt: P51397), aa 77 - 102.
Positive control
NIH3T3 cell lysate, Hela cell lysate and human stomach carcinoma
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
E59
Isotype
IgG
Research areas
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/250.
IHC-P: 1/100 - 1/250.
IP: 1/50.
WB: 1/10000. Detects a band of approximately 11 kDa.
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in mediating interferon-gamma-induced cell death.
Target information above from: UniProt accessionP51397
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAP 1 antibody
- Dap antibody
- DAP-1 antibody
see all
Database links
- Entrez Gene: 1611 Human
- Omim: 600954 Human
- SwissProt: P51397 Human
- Unigene: 75189 Human
Anti-DAP1 antibody [E59] images:
Western blot - DAP1 antibody [E59] (ab32056)
![Western blot - DAP1 antibody [E59] (ab32056)](/ps/datasheet/Images/32/ab32056/ab32056-1.JPG)
Anti-DAP1 antibody [E59] (ab32056) at 1/10000 dilution + NIH3T3 cell lysate
Observed band size : 11 kDa (why is the actual band size different from the predicted?)
Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence - DAP1 antibody [E59] (ab32056)
![Immunofluorescence - DAP1 antibody [E59] (ab32056)](/ps/datasheet/Images/32/ab32056/ab32056-2.JPG)
Immunofluorescent analysis of DAP1 expression in HeLa cells using 1/250 ab32056.
Immunohistochemistry (Paraffin-embedded sections) - DAP1 antibody [E59] (ab32056)
![Immunohistochemistry (Paraffin-embedded sections) - DAP1 antibody [E59] (ab32056)](/ps/datasheet/Images/32/ab32056/ab32056-3.JPG)
ab32056, at a 1/100 dilution, staining human stomach carcinoma by immunohistochemistry, Paraffin embedded tissue.
References for Anti-DAP1 antibody [E59] (ab32056)
ab32056 has not yet been referenced specifically in any publications.
Publishing research using ab32056? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"