![Western blot - DAP3 antibody [42C617] (ab11928) image](#DAP3-Primary-antibodies-ab11928-4.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-DAP3 antibody [42C617]
See all DAP3 products (5) ...
Description
Mouse monoclonal [42C617] to DAP3
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide:corresponding to amino acids 45-52 and 358-371 of Human DAP3.
QYYLENNWLQ HEKA
Positive control
Jurkat whole-cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
42C617
Isotype
IgG1
Research areas
Cell Biology >> Apoptosis >> Mitochondrial
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Western blot - DAP3 antibody [42C617] (ab11928)
![Western blot - DAP3 antibody [42C617] (ab11928) image](/ps/datasheet/images/11/ab11928/DAP3-Primary-antibodies-ab11928-4.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 45 kDa.
Target
Function
Involved in mediating interferon-gamma-induced cell death.
Tissue specificity
Ubiquitous.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP51398
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- 28S ribosomal protein S29 antibody
- 28S ribosomal protein S29 mitochondrial antibody
see all
Database links
- Entrez Gene: 7818 Human
- Entrez Gene: 65111 Mouse
- Omim: 602074 Human
- SwissProt: P51398 Human
- SwissProt: Q9ER88 Mouse
- Unigene: 516746 Human
- Unigene: 29028 Mouse
- Unigene: 400397 Mouse
Anti-DAP3 antibody [42C617] images:
Western blot - DAP3 antibody [42C617] (ab11928)
Anti-DAP3 antibody [42C617] (ab11928) at 2 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
References for Anti-DAP3 antibody [42C617] (ab11928)
This product has been referenced in:
- Rorbach Jet al. PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 21666256) »
- Dennerlein Set al. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. Biochem J 430:551-8 (2010).Read more (PubMed: 20604745) »
See all 3 publications for this product
Publishing research using ab11928? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"