DAP3 antibody (ab2555)

Product Name 

DAP3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to DAP3

Immunogen 

Synthetic peptide surrounding amino acid Lys58

Reacts with 
(species key)

Hu

Specificity 

This antibody recognizes the 46 kDa human DAP 3.
It does not cross-react with other DAP family members.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 - 2 µg/ml.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Mitochondrial

Research areas 

Cell Biology >> Apoptosis >> Mitochondrial
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome

Relevance 

Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for DAP3 antibody (ab2555)

  This product has been used in: (two most recent references)

Hulkko SM et al. The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the glucocorticoid receptor and affects the receptor function. Biochem J 349 Pt 3:885-93 (2000). PubMed: 10903152

Kissil JL et al. Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. J Biol Chem 270:27932-6 (1995). PubMed: 7499268
If you publish research using ab2555 please let us know so that we can cite the reference on this datasheet.

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