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Product Name
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DAP3 antibody
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See all DAP3 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to DAP3
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Immunogen
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Synthetic peptide: NPSLLERHCAYL, corresponding to C terminal amino acids 387-398 of Human DAP3.
BLAST 'NPSLLERHCAYL' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is expected to recognise both human isoforms of this protein.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Western Blot – Approx 40-45kDa band seen in HeLa lysate.
Recommended for use at 2.0-4.0µg/ml.
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Positive control
(see definition)
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HeLa lysate
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Cellular localization
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Mitochondrial
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Research areas
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Cell Biology >> Apoptosis >> Mitochondrial
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Relevance
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Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DAP3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to DAP3
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DAP3 antibody - more information
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