Overview

  • Product nameAnti-DAP5 antibodySee all DAP5 primary antibodies ...
  • Description
    Rabbit polyclonal to DAP5
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 204 of Human DAP5 (NP_001409)

  • Positive control
    • A431 whole cell lysate and Cal27. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab97302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 102 kDa.
IHC-P 1/100.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionAppears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
  • Tissue specificityUbiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
  • Sequence similaritiesBelongs to the eIF4G family.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • Post-translational
    modifications
    Phosphorylation; hyperphosphorylated during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAP-5 antibody
    • Death associated protein 5 antibody
    • Death-associated protein 5 antibody
    • eIF-4-gamma 2 antibody
    • eIF-4G 2 antibody
    • eIF4G 2 antibody
    • EIF4G2 antibody
    • Eukaryotic translation initiation factor 4 gamma 2 antibody
    • IF4G2_HUMAN antibody
    • Nat1 antibody
    • Novel APOBEC-1 target 1 antibody
    • p97 antibody
    • Translation repressor NAT1 antibody
    see all

Anti-DAP5 antibody images

  • ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-DAP5 antibody (ab97302) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 102 kDa
    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Cal27, using ab97302 at 1/100 dilution

References for Anti-DAP5 antibody (ab97302)

ab97302 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"