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Western blot - DAPP1 antibody (ab63349)
Western blot - DAPP1 antibody (ab63349)
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Product Name 

DAPP1 antibody

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to DAPP1

Immunogen 

Synthesized non phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (SIYPES)

Reacts with 
(species key)

Hu, Ms

Specificity 

This antibody detects endogenous levels of total DAPP1 protein.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts from 293 cells, treated with Insulin

Cellular localization 

Cell Membrane and Cytoplasmic. Membrane associated after cell stimulation leading to its translocation.

Research areas 

Signal Transduction >> Adapters >> Cytoplasmic
Immunology >> Adaptive Immunity >> B Cells >> Non-CD

Relevance 

This protein may act as a B cell associated adapter that regulates B cell antigen receptor (BCR) signaling downstream of PI3K.
Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B lymphocytes, but not T lymphocytes or nonhematopoietic cells.

 

Alternative names DAPP1 antibody (ab63349)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

27071    (Human)
26377    (Mouse)

NCBI

    

NP_055210    (Human)

Omim

    

605768    (Human)

SwissProt

    

Q9UN19    (Human)
Q9QXT1    (Mouse)

Unigene

    

436271    (Human)
708484    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

Purified from rabbit antiserum.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DAPP1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for DAPP1 antibody images, references, products related to ab63349 and other tools.

 

DAPP1 antibody images:

Product image  Western blot - DAPP1 antibody (ab63349)

Western blot - DAPP1 antibody (ab63349)

All lanes : DAPP1 antibody (ab63349) at 1/500 dilution

Lane 1 : extracts from 293 cells, treated with Insulin (0.01U/ml, 2mins), without immunising peptide
Lane 2 : extracts from 293 cells, treated with Insulin (0.01U/ml, 2mins), with immunising peptide


Predicted band size : 32 kDa
Observed band size : 32 kDa
Additional bands at : 117 kDa. We are unsure as to the identity of these extra bands.

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

 
 

Search PubMed (MEDLINE) for references to DAPP1

 

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