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Products:Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Overview
Product name
Anti-DAPP1 antibody
See all DAPP1 products (5) ...
Description
Rabbit polyclonal to DAPP1
Specificity
This antibody detects endogenous levels of total DAPP1 protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthesized non phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (SIYPES)
Positive control
Extracts from 293 cells, treated with Insulin
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Adapters >> Cytoplasmic
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
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Western blot - DAPP1 antibody (ab63349)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
WB: 1/500 - 1/1000.Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Target
Function
May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
Tissue specificity
Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
Sequence similarities
Contains 1 PH domain.
Contains 1 SH2 domain.
Post-translational
modifications
Phosphorylated on tyrosine residues.
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Target information above from: UniProt accessionQ9UN19
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp667E0716 antibody
- B cell adapter molecule of 32 kDa antibody
- B lymphocyte adapter protein Bam32 antibody
see all
Database links
- Entrez Gene: 27071 Human
- Entrez Gene: 26377 Mouse
- NCBI: 7657007 Human
- Omim: 605768 Human
- SwissProt: Q9UN19 Human
- SwissProt: Q9QXT1 Mouse
- Unigene: 436271 Human
- Unigene: 708484 Human
see all
Anti-DAPP1 antibody images:
Western blot - DAPP1 antibody (ab63349)
All lanes : Anti-DAPP1 antibody (ab63349) at 1/500 dilution
Lane 1 : extracts from 293 cells, treated
with Insulin (0.01U/ml, 2mins), without immunising peptide
Lane 2 : extracts from 293 cells, treated
with Insulin (0.01U/ml, 2mins), with immunising peptide
Predicted band size : 32 kDa
Observed band size : 32 kDa
Additional bands at : 117 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
References for Anti-DAPP1 antibody (ab63349)
ab63349 has not yet been referenced specifically in any publications.
Publishing research using ab63349? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"