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Anti-DAPP1 antibody (ab63349)

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Overview

Product name

Anti-DAPP1 antibody
See all DAPP1 products (5) ...

Description

Rabbit polyclonal to DAPP1

Specificity

This antibody detects endogenous levels of total DAPP1 protein.

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthesized non phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (SIYPES)

Positive control

Extracts from 293 cells, treated with Insulin

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from rabbit antiserum.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - DAPP1 antibody (ab63349)Western blot - DAPP1 antibody (ab63349) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab63349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).

Target

Function

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.

Tissue specificity

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.

Sequence similarities

Contains 1 PH domain.
Contains 1 SH2 domain.

Post-translational
modifications

Phosphorylated on tyrosine residues.

Cellular localization

Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.

Target information above from: UniProt accessionQ9UN19 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DKFZp667E0716 antibody
  • B cell adapter molecule of 32 kDa antibody
  • B lymphocyte adapter protein Bam32 antibody
  • B-cell adapter molecule of 32 kDa antibody
  • BAM32 antibody
  • DAPP1 antibody
  • DAPP1_HUMAN antibody
  • Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody
  • Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody
  • hDAPP1 antibody
see all

Anti-DAPP1 antibody images:

  Western blot - DAPP1 antibody (ab63349)

Western blot - DAPP1 antibody (ab63349)

All lanes : Anti-DAPP1 antibody (ab63349) at 1/500 dilution

Lane 1 : extracts from 293 cells, treated with Insulin (0.01U/ml, 2mins), without immunising peptide
Lane 2 : extracts from 293 cells, treated with Insulin (0.01U/ml, 2mins), with immunising peptide


Predicted band size : 32 kDa
Observed band size : 32 kDa
Additional bands at : 117 kDa. We are unsure as to the identity of these extra bands.

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

References for Anti-DAPP1 antibody (ab63349)

ab63349 has not yet been referenced specifically in any publications.

Publishing research using ab63349? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"