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Anti-DAPP1 (phospho Y139) antibody (ab63396)

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Overview

Product name

Anti-DAPP1 (phospho Y139) antibody
See all DAPP1 products (5) ...

Description

Rabbit polyclonal to DAPP1 (phospho Y139)

Specificity

ab63396 detects endogenous levels of DAPP1 only when phosphorylated at tyrosine 139.

Tested applications

ICC/IF, WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic phosphopeptide derived from human DAPP1 around the phosphorylation site of tyrosine 139 (S-I-YP-E-S).

Positive control

WB: 293 cell extracts treated with Insulin (0.01U/ml, 2mins). ICC/IF: A549 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab63396 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Adapters >> Cytoplasmic
Immunology >> Adaptive Immunity >> B Cells >> Non-CD

  • Immunocytochemistry/ Immunofluorescence - DAPP1 (phospho Y139) antibody (ab63396)Immunocytochemistry/ Immunofluorescence - DAPP1 (phospho Y139) antibody (ab63396) image (enlarge)

  • Western blot - DAPP1 (phospho Y139) antibody (ab63396)Western blot - DAPP1 (phospho Y139) antibody (ab63396) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab63396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    ICC/IF

     ICC/IF: 1/500 - 1/1000.

    ICC/IF: 1/500 - 1/1000.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 32 kDa.

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

Target

Function

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.

Tissue specificity

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.

Sequence similarities

Contains 1 PH domain.
Contains 1 SH2 domain.

Post-translational
modifications

Phosphorylated on tyrosine residues.

Cellular localization

Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.

Target information above from: UniProt accessionQ9UN19 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DKFZp667E0716 antibody
  • B cell adapter molecule of 32 kDa antibody
  • B lymphocyte adapter protein Bam32 antibody
  • B-cell adapter molecule of 32 kDa antibody
  • BAM32 antibody
  • DAPP1 antibody
  • DAPP1_HUMAN antibody
  • Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody
  • Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody
  • hDAPP1 antibody
see all

Database links

see all

Anti-DAPP1 (phospho Y139) antibody images:

  Immunocytochemistry/ Immunofluorescence - DAPP1 (phospho Y139) antibody (ab63396)

Immunocytochemistry/ Immunofluorescence - DAPP1 (phospho Y139) antibody (ab63396)

ab63396, at 1/500 dilution, staining DAPP1 in A549 cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.

  Western blot - DAPP1 (phospho Y139) antibody (ab63396)

Western blot - DAPP1 (phospho Y139) antibody (ab63396)

All lanes : Anti-DAPP1 (phospho Y139) antibody (ab63396) at 1/500 dilution

Lane 1 : 293 cell extracts treated with Insulin (0.01U/ml, 2mins)
Lane 2 : 293 cell extracts treated with Insulin (0.01U/ml, 2mins) with immunising peptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 32 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DAPP1 (phospho Y139) antibody (ab63396)

ab63396 has not yet been referenced specifically in any publications.

Publishing research using ab63396? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"