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Western blot - DARC antibody (ab40821)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DARC antibody (ab40821)
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Product Name
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DARC antibody
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See all DARC antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to DARC
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Immunogen
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Synthetic peptide: C-HRAELSPSTENSSQLDFED, corresponding to N terminal amino acids 6-24 of Human DARC
BLAST 'C-HRAELSPSTENSSQLDFED' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp, Glla, HGbbn
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, Flow Cyt, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/16000.
Flow Cyt: Use at an assay dependent concentration (see Abreview for further details published on 3rd April, 2007).
IHC-P: Use at a concentration of 3 µg/ml.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Kidney and Human Liver lysates.
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Cellular localization
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Membrane; multi-pass membrane protein
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Research areas
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Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Microbiology >> Organism >> Eukaryotic Microorganism >> Protozoa >> Plasmodium
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
Cardiovascular >> Blood >> Other
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Relevance
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DARC is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
Polymorphisms in this gene are the basis of the Duffy blood group system.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DARC antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DARC antibody images, references, products related to ab40821 and other tools.
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DARC antibody images:
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 Western blot - DARC antibody (ab40821)
DARC antibody (ab40821) at 0.1 µg/ml + Human Liver lysate - (35µg protein in RIPA buffer).
Secondary
anti-goat-HRP at 1/3000 dilution
Predicted band size : 36 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DARC antibody (ab40821)
ab40821 (3µg/ml) staining Human Kidney by IHC-P. Microwaved antigen retrieval with citrate buffer (pH 6), HRP-staining.
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Search PubMed (MEDLINE) for references to DARC
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DARC antibody - more information
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Customer reviews (feedback) regarding DARC antibody |
Customer FAQs regarding DARC antibody |
Protocols for DARC antibody |
Price and availability of products related to DARC antibody |
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DARC antibody - related products:
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DARC antibody - other tools:
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Contact Abcam with a Technical Enquiry about DARC antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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