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Products:Cardiovascular >> Blood >> Blood Cell Antigens
Overview
Product name
Anti-DARC antibody
See all DARC products (5) ...
Description
Goat polyclonal to DARC
Tested applications
Flow Cyt, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee, Gorilla, Hoolock Gibbon
Immunogen
Synthetic peptide: C-HRAELSPSTENSSQLDFED, corresponding to N terminal amino acids 6-24 of Human DARC
C-HRAELSPS TENSSQLDFE D
Positive control
Human kidney and human liver lysates. Human kidney tissue sections.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Microbiology >> Organism >> Eukaryotic Microorganism >> Protozoa >> Plasmodium
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Blood Cell Antigens
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution. ((see Abreview for further details published on 3rd April, 2007).)
IHC-P: Use a concentration of 3 µg/ml
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
Target
Function
Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
Tissue specificity
Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
Sequence similarities
Belongs to the G-protein coupled receptor Duffy family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ16570
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCBP1 antibody
- CD234 antibody
- CD234 antigen antibody
see all
Database links
- Entrez Gene: 2532 Human
- Omim: 110700 Human
- SwissProt: Q16570 Human
- Unigene: 153381 Human
Anti-DARC antibody images:
Western blot - DARC antibody (ab40821)
Anti-DARC antibody (ab40821) at 0.1 µg/ml + Human liver lysate - (35µg protein in RIPA buffer).
Secondary
anti-goat-HRP at 1/3000 dilution
Predicted band size : 36 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DARC antibody (ab40821)
ab40821 at 3µg/ml staining DARC in human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Microwaved antigen retrieval using citrate buffer pH 6 was performed, HRP-staining.This antobody stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on human lung tissue showing endothelial staining.
References for Anti-DARC antibody (ab40821)
ab40821 has not yet been referenced specifically in any publications.
Publishing research using ab40821? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"