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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab58965? Please let us know so that we can cite the reference in this datasheet
ab58965 has been referenced in 1 publications.
- Wessels JM et al. Expression of chemokine decoy receptors and their ligands at the porcine maternal-fetal interface. Immunol Cell Biol : (2010). PubMed: 20680026
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - DARC antibody (ab58965)
Lane 1 : Anti-DARC antibody (ab58965) at 0.5 µg/ml
Lane 2 : Anti-DARC antibody (ab58965) at 1 µg/ml
Lane 3 : Anti-DARC antibody (ab58965) at 2 µg/ml
Lane 1 : mouse brain tissue lysate
Lane 2 : mouse brain tissue lysate
Lane 3 : mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
Lanes 1 - 2 : Anti-rabbit IgG HRP at 1/1000 dilution
Lane 3 : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence-DARC antibody(ab58965)
ICC/IF image of ab58965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58965, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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