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I need to know what epitope the Anti-DARC antibody (ab58965) recognizes. |
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ANSWER: |
Thank you very much for your inquiry.
However, the peptide sequences used to produce this antibody is proprietary information and cannot be disclosed at this time.
I am sorry that I cannot help you further in this matter but please let me know if there is anything else I can help you wit |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Anti-DARC antibody (ab58965) at 0.5 µg/ml
Lane 2 : Anti-DARC antibody (ab58965) at 1 µg/ml
Lane 3 : Anti-DARC antibody (ab58965) at 2 µg/ml
Lane 1 : mouse brain tissue lysate
Lane 2 : mouse brain tissue lysate
Lane 3 : mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
Lanes 1 - 2 : Anti-rabbit IgG HRP at 1/1000 dilution
Lane 3 : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab58965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58965, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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