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Products:Cardiovascular >> Blood >> Blood Cell Antigens
Overview
Product name
Anti-DARC antibody
See all DARC products (5) ...
Description
Rabbit polyclonal to DARC
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A 16 amino acid peptide from near the carboxy terminus of human DARC.
Positive control
WB: Mouse brain tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab58965is purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Microbiology >> Organism >> Eukaryotic Microorganism >> Protozoa >> Plasmodium
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
Cardiovascular >> Blood >> Other
Cardiovascular >> Blood >> Blood Cell Antigens
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1.0 - 2.0 µg/ml.Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Target
Function
Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
Tissue specificity
Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
Sequence similarities
Belongs to the G-protein coupled receptor Duffy family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ16570
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCBP1 antibody
- CD234 antibody
- CD234 antigen antibody
see all
Database links
- Entrez Gene: 2532 Human
- Entrez Gene: 13349 Mouse
- Entrez Gene: 681489 Rat
- Omim: 110700 Human
- SwissProt: Q16570 Human
- SwissProt: Q9QUI6 Mouse
- Unigene: 153381 Human
- Unigene: 6393 Mouse
Anti-DARC antibody images:
Western blot - DARC antibody (ab58965)
Lane 1 : Anti-DARC antibody (ab58965) at 0.5 µg/ml
Lane 2 : Anti-DARC antibody (ab58965) at 1 µg/ml
Lane 3 : Anti-DARC antibody (ab58965) at 2 µg/ml
Lane 1 : mouse brain tissue lysate
Lane 2 : mouse brain tissue lysate
Lane 3 : mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
Lanes 1 - 2 : Anti-rabbit IgG HRP at 1/1000 dilution
Lane 3 : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence-DARC antibody(ab58965)
ICC/IF image of ab58965 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-DARC antibody (ab58965)
This product has been referenced in:
- Wessels JMet al. Expression of chemokine decoy receptors and their ligands at the porcine maternal-fetal interface. Immunol Cell Biol : (2010).Read more (PubMed: 20680026) »
See 1 publication for this product
Publishing research using ab58965? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"