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Western blot - DARC antibody (ab58965)
(enlarge)
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Product Name
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DARC antibody
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See all DARC antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DARC
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Immunogen
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A 16 amino acid peptide from near the carboxy terminus of human DARC.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1.0 - 2.0 µg/ml. Detects bands of approximately 23 and 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Mouse brain tissue lysate.
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Cellular localization
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Membrane; multi-pass membrane protein
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Research areas
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Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Microbiology >> Organism >> Eukaryotic Microorganism >> Protozoa >> Plasmodium
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokine Rec. (CCR)
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokine Rec. (CXCR)
Cardiovascular >> Blood >> Other
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Relevance
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DARC is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
Polymorphisms in this gene are the basis of the Duffy blood group system.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab58965is purified.
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DARC antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DARC antibody images, references, products related to ab58965 and other tools.
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DARC antibody images:
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 Western blot - DARC antibody (ab58965)
Lane 1 : DARC antibody (ab58965) at 0.5 µg/ml
Lane 2 : DARC antibody (ab58965) at 1 µg/ml
Lane 3 : DARC antibody (ab58965) at 2 µg/ml
Lane 1 : mouse brain tissue lysate
Lane 2 : mouse brain tissue lysate
Lane 3 : mouse brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
Lanes 1 - 2 : Anti-rabbit IgG HRP at 1/1000 dilution
Lane 3 : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to DARC
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DARC antibody - more information
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Customer reviews (feedback) regarding DARC antibody |
Customer FAQs regarding DARC antibody |
Protocols for DARC antibody |
Price and availability of products related to DARC antibody |
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DARC antibody - related products:
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DARC antibody - other tools:
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Contact Abcam with a Technical Enquiry about DARC antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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