![Western blot - DARPP32 antibody [EP721Y] (ab40802) image](#ab40802 wb.bmp)
![Immunohistochemistry (Paraffin-embedded sections) - DARPP32 antibody [EP721Y] (ab40802) image](#ab40802 ihc.bmp)
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Product name
Anti-DARPP32 antibody [EP721Y]
See all DARPP32 products (11) ...
Description
Rabbit monoclonal [EP721Y] to DARPP32
Tested applications
WB, IHC-P, ICC, IPmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues in the C-term of human DARPP32.
Positive control
Human brain lysate and human gastric adenocarcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP721Y
Isotype
IgG
Research areas
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Dopamine Receptors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC: 1/50 - 1/100.
IHC-P: 1/250 - 1/500.
IP: 1/50.
WB: 1/5000 - 1/20000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Inhibitor of protein-phosphatase 1.
Sequence similarities
Belongs to the protein phosphatase inhibitor 1 family.
Post-translational
modifications
Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UD71
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
see all
Database links
- Entrez Gene: 84152 Human
- Omim: 604399 Human
- SwissProt: Q9UD71 Human
- Unigene: 286192 Human
Anti-DARPP32 antibody [EP721Y] images:
Western blot - DARPP32 antibody [EP721Y] (ab40802)
![Western blot - DARPP32 antibody [EP721Y] (ab40802)](/ps/datasheet/Images/40/ab40802/ab40802 wb.bmp)
Anti-DARPP32 antibody [EP721Y] (ab40802) at 1/20000 dilution + Human brain lysate at 10 µg
Predicted band size : 32 kDa
Observed band size : 32 kDa
Immunohistochemistry (Paraffin-embedded sections) - DARPP32 antibody [EP721Y] (ab40802)
![Immunohistochemistry (Paraffin-embedded sections) - DARPP32 antibody [EP721Y] (ab40802)](/ps/datasheet/Images/40/ab40802/ab40802 ihc.bmp)
Immunohistochemical analysis human gastric adenocarcinoma using ab40802.
(dilution 1:250)
References for Anti-DARPP32 antibody [EP721Y] (ab40802)
ab40802 has not yet been referenced specifically in any publications.
Publishing research using ab40802? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"