Anti-DARS antibody - C-terminal (ab71436)

Overview

• Product nameAnti-DARS antibody - C-terminalSee all DARS primary antibodies ...
• Description
  Rabbit polyclonal to DARS - C-terminal
• Tested applicationsIHC-P, ELISA, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide from the C terminal region of human DARS conjugated to KLH

• Positive controlK562 cell line lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Purification notesab71436 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • Translation
  • tRNA synthetase

Applications

Target

• FunctionCatalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
• Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession P14868 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1615 Human
  • Omim: 603084 Human
  • SwissProt: P14868 Human
  • Unigene: 503787 Human

• Alternative names
  Aspartate tRNA ligase 1 cytoplasmic antibodyAspartate tRNA ligase antibodyAspartate--tRNA ligase antibody

  Aspartyl tRNA synthetase antibodyAspartyl tRNA synthetase cytoplasmic antibodyAspartyl-tRNA synthetase antibodyAspRS antibodyCell proliferation inducing gene 40 protein antibodyCell proliferation inducing protein 40 antibodyCell proliferation-inducing gene 40 protein antibodycytoplasmic antibodyDars antibodyDKFZp781B11202 antibodyMGC111579 antibodySYDC_HUMAN antibody

  see all

Anti-DARS antibody - C-terminal images

• 

  Western blot - DARS antibody - C-terminal (ab71436)
  Anti-DARS antibody - C-terminal (ab71436) at 1/60 dilution + K562 cell line lysate at 35 µg
  
  Predicted band size : 57 kDa
  Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DARS antibody - C-terminal (ab71436)
  ab71436 at 1/50 dilution staining DARS C - terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.