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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-DARS antibody - C-terminal
See all DARS products (4) ...
Description
Rabbit polyclonal to DARS - C-terminal
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C terminal region of human DARS conjugated to KLH
Positive control
K562 cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
ab71436 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/50 - 1/100.Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
Target
Function
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP14868
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytoplasmic antibody
- Aspartate tRNA ligase 1 cytoplasmic antibody
- Aspartate tRNA ligase antibody
see all
Database links
- Entrez Gene: 1615 Human
- Omim: 603084 Human
- SwissProt: P14868 Human
- Unigene: 503787 Human
Anti-DARS antibody - C-terminal images:
Western blot - DARS antibody - C-terminal (ab71436)
Anti-DARS antibody - C-terminal (ab71436) at 1/60 dilution + K562 cell line lysate at 35 µg
Predicted band size : 57 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DARS antibody - C-terminal (ab71436)
ab71436 at 1/50 dilution staining DARS C - terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-DARS antibody - C-terminal (ab71436)
ab71436 has not yet been referenced specifically in any publications.
Publishing research using ab71436? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"