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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Anti-DARS antibody - C-terminal
See all DARS products (4) ...
Rabbit polyclonal to DARS - C-terminal
IHC-P, ELISA, WBmore details
Reacts with
Human
Synthetic peptide from the C terminal region of human DARS conjugated to KLH
K562 cell line lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration information loading...
Ammonium Sulphate Precipitation
ab71436 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Our Abpromise guarantee covers the use of ab71436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
ELISA: 1/1000
WB: 1/50 - 1/100.Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Belongs to the class-II aminoacyl-tRNA synthetase family.
Cytoplasm.
Target information above from: UniProt accessionP14868
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - DARS antibody - C-terminal (ab71436)

Anti-DARS antibody - C-terminal (ab71436) at 1/60 dilution + K562 cell line lysate at 35 µg
Predicted band size : 57 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DARS antibody - C-terminal (ab71436)

ab71436 at 1/50 dilution staining DARS C - terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
ab71436 has not yet been referenced specifically in any publications.
Publishing research using ab71436? Please let us know so that we can cite the reference in this datasheet
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Anti-DARS antibody - C-terminal (ab71436) at 1/60 dilution + K562 cell line lysate at 35 µg
Predicted band size : 57 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)

ab71436 at 1/50 dilution staining DARS C - terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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