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Overview

  • Product nameDARS protein (His tag)
  • Protein descriptionRecombinant full length Human DARS (amino acids 1-501) with N terminal His tag; 521 amino acids with tag; Predicted MWt 59.3 kDa (UniProt P14868).
  • Uniprot accessionP14868
  • Molecular weight59.300kDa inclusive of tags
  • Protein length501 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 85 % by SDS-PAGE
  • Purification notesab124568 was purified using conventional chromatography techniques.
  • E.C. Number6.1.1.12
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 40% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Sequence notesMGSSHHHHHHSSGLVPRGSHMPSASASRKSQEKPREIMDA AEDYAKERYGISSMIQSQEKPDRVLVRVRDLTIQKADEVV WVRARVHTSRAKGKQCFLVLRQQQFNVQALVAVGDHASKQ MVKFAANINKESIVDVEGVVRKVNQKIGSCTQQDVELHVQ KIYVISLAEPRLPLQLDDAVRPEAEGEEEGRATVNQDTRL DNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQT PKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICA DFEKVFSIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYH EVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFL EPTLRLEYCEALAMLREAGVEMGDEDDLSTPNEKLLGHLV KEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQSNSYDMFM RGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFR FGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLT P
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab124568 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.
  • Application notesThis peptide can be used with studies using ab151974.

    • Protein info

    • Alternative names
        Aspartate tRNA ligaseAspartate tRNA ligase 1 cytoplasmicAspartate--tRNA ligase
        Aspartyl tRNA synthetaseAspartyl tRNA synthetase cytoplasmicAspartyl-tRNA synthetaseAspRSCell proliferation inducing gene 40 proteinCell proliferation inducing protein 40Cell proliferation-inducing gene 40 proteincytoplasmicDarsDKFZp781B11202MGC111579SYDC_HUMAN
      see all
  • FunctionCatalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
  • Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P14868 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    DARS protein (His tag) images

    • SDS-PAGE - DARS protein (His tag) (ab124568)
      SDS-PAGE - DARS protein (His tag) (ab124568)
      15% SDS-PAGE analysis of 3 µg ab124568.

    References for DARS protein (His tag) (ab124568)

    ab124568 has not yet been referenced specifically in any publications.

    Publishing research using ab124568 ? Please let us know so that we can cite the reference in this datasheet

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