![Western blot - DAZAP1 antibody [2242C2a] (ab76578) image](#ab76578.gif)
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Overview
Product name
Anti-DAZAP1 antibody [2242C2a]
See all DAZAP1 products (3) ...
Description
Mouse monoclonal [2242C2a] to DAZAP1
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment comprising of 50-200 amino acids from near the C terminus of Human DAZAP1 (NM_170711).
Positive control
Recombinant fragment of human DAZAP1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS,(8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab76578 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Clonality
Monoclonal
Clone number
2242C2a
Isotype
IgG2b
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
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Western blot - DAZAP1 antibody [2242C2a] (ab76578)
![Western blot - DAZAP1 antibody [2242C2a] (ab76578) image](/ps/datasheet/Images/76/ab76578/ab76578.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.2 - 2 µg/ml.Detects a band of approximately 36 kDa (predicted molecular weight: 41 kDa).
Dot: Use at an assay dependent dilution.
Target
Function
RNA-binding protein, which may be required during spermatogenesis.
Tissue specificity
Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
Target information above from: UniProt accessionQ96EP5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAZ associated protein 1 antibody
- DAZ-associated protein 1 antibody
- Dazap1 antibody
see all
Database links
- Entrez Gene: 26528 Human
- Omim: 607430 Human
- SwissProt: Q96EP5 Human
- Unigene: 222510 Human
Anti-DAZAP1 antibody [2242C2a] images:
Western blot - DAZAP1 antibody [2242C2a] (ab76578)
Anti-DAZAP1 antibody [2242C2a] (ab76578) at 2 µg/ml + immunising recombinant protein
Predicted band size : 41 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used
References for Anti-DAZAP1 antibody [2242C2a] (ab76578)
ab76578 has not yet been referenced specifically in any publications.
Publishing research using ab76578? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"