Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Overview
Product name
Anti-DAZAP1 antibody
See all DAZAP1 products (3) ...
Description
Rabbit polyclonal to DAZAP1
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
A region within synthetic peptide: QAAPDMSKPP TAQPDFPYGQ YGLGSYSPAP PGCGPHFVYS LMVRLSSDVA, corresponding to amino acids 240-289 of Human DAZAP1
QAAPDMSKPP TAQPDFPYGQ YGLGSYSPAP PGCGPHFVYS LMVRLSSDVA
Positive control
HepG2 cell lysate, Human kidney.
Properties
Form
Lyophilised:
Add 50 µl of distilled water for a final concnetration of 1 mg/ml.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Titre using peptide based assay 1/312500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
RNA-binding protein, which may be required during spermatogenesis.
Tissue specificity
Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
Target information above from: UniProt accessionQ96EP5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAZ associated protein 1 antibody
- DAZ-associated protein 1 antibody
- Dazap1 antibody
see all
Database links
- Entrez Gene: 26528 Human
- Omim: 607430 Human
- SwissProt: Q96EP5 Human
- Unigene: 222510 Human
Anti-DAZAP1 antibody images:
Western blot - DAZAP1 antibody (ab51010)
Lane 1 : Marker
Lane 2 : Anti-DAZAP1 antibody (ab51010) at 0.5 µg/ml
Lane 1 : As above
Lane 2 : HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 43 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Gel concnetration: 12%
Immunohistochemistry (Paraffin-embedded sections) - DAZAP1 antibody (ab51010)
Ab51010 at 4.0 µg/ml staining epithelial cells of renal tubule from human kidney; paraffin embedded; magnification 400x.
References for Anti-DAZAP1 antibody (ab51010)
This product has been referenced in:
- Hayakawa Het al. Human proteins that specifically bind to 8-oxoguanine-containing RNA and their responses to oxidative stress. Biochem Biophys Res Commun 403:220-4 (2010). WB; Human.Read more (PubMed: 21073862) »
See 1 publication for this product
Publishing research using ab51010? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"