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Western blot - DAZL antibody (ab34139)
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Immunocytochemistry/ Immunofluorescence - DAZL antibody (ab34139)
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Immunocytochemistry/ Immunofluorescence - DAZL antibody (ab34139)
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Product Name
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DAZL antibody
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See all DAZL antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DAZL
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse DAZL. (Peptide available as ab27733.)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 0.5 - 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Testis (Human) Whole Tissue Lysate and Testis (Mouse) Whole Tissue Lysate.
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Cellular localization
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Predominantly cytoplasmic. Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onwards.
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Research areas
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Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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DAZL is a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localised to the cytoplasm.
Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.210 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DAZL antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DAZL antibody images, references, products related to ab34139 and other tools.
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DAZL antibody images:
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 Western blot - DAZL antibody (ab34139)
All lanes : DAZL antibody (ab34139) at 1 µg/ml
Lane 1 : Testis (Human) Whole Tissue Lysate
Lane 2 : Testis (Mouse) Whole Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 33 kDa
The difference in the observed band size of DAZL between the human and mouse samples is thought to be due to species-dependent post-translational modification as the proteins are not 100% identical between species.
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Immunocytochemistry/ Immunofluorescence - DAZL antibody (ab34139)
ab34139 at 1/100 staining human blastocyst cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor ® 488 conjugated secondary antibody was used. This image is courtesy of an Abreview submitted by Dr Sharon Sneddon
See Abreview
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Immunocytochemistry/ Immunofluorescence - DAZL antibody (ab34139)
The image, submitted as part of an Abreview, shows DAZL staining (green) of a human blastocyst. The antibody stains the cytoplasm of cells of the inner cell mass and trophoblast. DAZL is known to be located to the cytoplasm and DAZL transcripts have previously been discovered in the inner cell mass and trophblast (see PMID: 15879466).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAZL antibody (ab34139)
The image shows staining of human testis tissue. The cytoplasm of Leydig cells and other cells in the seminiferous tubules stained positive. The antigen retrieval techniques used were Citrate at pH6 or Tris EDTA at pH9.
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Search PubMed (MEDLINE) for references to DAZL
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DAZL antibody - more information
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Customer reviews (feedback) regarding DAZL antibody |
Customer FAQs regarding DAZL antibody |
Protocols for DAZL antibody |
Price and availability of products related to DAZL antibody |
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DAZL antibody - related products:
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DAZL antibody - other tools:
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Contact Abcam with a Technical Enquiry about DAZL antibody |
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