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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-DAZL antibody
See all DAZL products (4) ...
Description
Rabbit polyclonal to DAZL
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Cow, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse DAZL.
(Peptide available as ab27733.)
Positive control
Testis (Human) Whole Tissue Lysate and Testis (Mouse) Whole Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 0.5 - 5 µg/ml.
ICC/IF: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Target
Function
RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
Tissue specificity
Testis specific.
Sequence similarities
Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain.
Domain
The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
Target information above from: UniProt accessionQ92904
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAZ homolog antibody
- DAZ like autosomal antibody
- DAZ-like autosomal antibody
see all
Database links
- Entrez Gene: 530116 Cow
- Entrez Gene: 1618 Human
- Entrez Gene: 13164 Mouse
- Omim: 601486 Human
- SwissProt: Q92904 Human
- SwissProt: Q64368 Mouse
- Unigene: 63695 Cow
- Unigene: 131179 Human
see all
Anti-DAZL antibody images:
Immunocytochemistry/ Immunofluorescence - DAZL antibody (ab34139)
ab34139 at 1/100 staining human blastocyst cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor ® 488 conjugated secondary antibody was used.
This image is courtesy of an Abreview submitted by Dr Sharon Sneddon
Immunocytochemistry/ Immunofluorescence - DAZL antibody (ab34139)
The image, submitted as part of an Abreview, shows DAZL staining (green) of a human blastocyst. The antibody stains the cytoplasm of cells of the inner cell mass and trophoblast. DAZL is known to be located to the cytoplasm and DAZL transcripts have previously been discovered in the inner cell mass and trophblast (see PMID: 15879466).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAZL antibody (ab34139)
The image shows staining of human testis tissue. The cytoplasm of Leydig cells and other cells in the seminiferous tubules stained positive. The antigen retrieval techniques used were Citrate at pH6 or Tris EDTA at pH9.
Western blot - DAZL antibody (ab34139)
All lanes : Anti-DAZL antibody (ab34139) at 1 µg/ml
Lane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lane 2 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 130 kDa,70 kDa,95 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DAZL antibody (ab34139)
IHC image of DAZL staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34139, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-DAZL antibody (ab34139)
This product has been referenced in:
- Chen Jet al. Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition. Genes Dev 25:755-66 (2011). WB; Mouse.Read more (PubMed: 21460039) »
- Rezende NCet al. Rex1 (Zfp42) null mice show impaired testicular function, abnormal testis morphology, and aberrant gene expression. Dev Biol 356:370-82 (2011). IHC-P; Mouse.Read more (PubMed: 21641340) »
See all 4 publications for this product
Publishing research using ab34139? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"