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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-DAZL antibody
See all DAZL products (4) ...
Description
Goat polyclonal to DAZL
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog
Immunogen
Synthetic peptide: GNGPQKKSVDR, corresponding to Internal sequence amino acids 240-250 of Human DAZL
GNGPQKKSVD R
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Western blot - DAZL antibody (ab40848)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Predicted molecular weight: 33 kDa.
Target
Function
RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
Tissue specificity
Testis specific.
Sequence similarities
Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain.
Domain
The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
Target information above from: UniProt accessionQ92904
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAZ homolog antibody
- DAZ like autosomal antibody
- DAZ-like autosomal antibody
see all
Database links
- Entrez Gene: 477055 Dog
- Entrez Gene: 1618 Human
- Entrez Gene: 13164 Mouse
- Entrez Gene: 680486 Rat
- Omim: 601486 Human
- SwissProt: Q92904 Human
- SwissProt: Q64368 Mouse
- Unigene: 131179 Human
see all
Anti-DAZL antibody images:
Western blot - DAZL antibody (ab40848)
Anti-DAZL antibody (ab40848) at 0.1 µg/ml + Human Ovary lysate (35µg protein in RIPA buffer).
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-DAZL antibody (ab40848)
ab40848 has not yet been referenced specifically in any publications.
Publishing research using ab40848? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"