|
|
|
|
Western blot - DAZL antibody (ab40848)
(enlarge)
|
|
|
|
|
Product Name
|
DAZL antibody
|
| |
See all DAZL antibodies (2)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Goat polyclonal to DAZL
|
|
Immunogen
|
Synthetic peptide: GNGPQKKSVDR, corresponding to Internal sequence amino acids 240-250 of Human DAZL
BLAST 'GNGPQKKSVDR' with  or 
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms, Rat, Dog
(Due to sequence homology)
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 33 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Predominantly cytoplasmic. Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onwards.
|
|
Research areas
|
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
|
|
Relevance
|
DAZL is a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localised to the cytoplasm.
Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Goat
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DAZL antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for DAZL antibody images, references, products related to ab40848 and other tools.
|
| |
DAZL antibody images:
|
 Western blot - DAZL antibody (ab40848)
DAZL antibody (ab40848) at 0.1 µg/ml + Human Ovary lysate (35µg protein in RIPA buffer).
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
|
| |
| |
|
Search PubMed (MEDLINE) for references to DAZL
|
| |
DAZL antibody - more information
|
Customer reviews (feedback) regarding DAZL antibody |
Customer FAQs regarding DAZL antibody |
Protocols for DAZL antibody |
Price and availability of products related to DAZL antibody |
| |
DAZL antibody - related products:
|
|
|
| |
DAZL antibody - other tools:
|
Contact Abcam with a Technical Enquiry about DAZL antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
