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Overview

  • Product nameDAZL proteinSee all DAZL proteins and peptides ...
  • Protein descriptionRecombinant full length Human DAZL (aa 1-296) with proprietary tag (M.Wt 58.19) (UniPort Q92904).
  • Uniprot accessionQ92904
  • Molecular weight58.190kDa inclusive of tags
  • Protein length296 amino acids
  • Expression hostWheat germ

    • Properties

  • Biological activityuseful for Antibody Production and Protein Array
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione
    Note: Reduced glutathione
  • Concentration information loading...
  • Additional notesBest use within three months from the date of receipt of this protein.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab112279 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Predicted molecular weight: 58.19 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. Ab112279 can be used as a WB positive control in conjunction with ab34139.
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.
    ELISA ELISA: Use at an assay dependent concentration.
    PepArr PepArr: Use at an assay dependent concentration.

    Protein info

    • Alternative names
        DAZ homologDAZ like autosomalDAZ-like autosomal
        DAZHDAZLDAZL 1DAZL_HUMANDAZL1DAZLADeleted in azoospermia likeDeleted in azoospermia like 1Deleted in azoospermia like autosomalDeleted in azoospermia-likeDeleted in azoospermia-like 1Germline specific RNA binding proteinMGC26406Spermatogenesis gene on the Y like autosomalSPGY like autosomalSPGY-like-autosomalSPGYLATpx2
      see all
  • FunctionRNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
  • Tissue specificityTestis specific.
  • Sequence similaritiesBelongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • DomainThe DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.

    • Target information above from: UniProt accession Q92904 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    DAZL protein images

    • SDS-PAGE - DAZL protein (ab112279)
      SDS-PAGE - DAZL protein (ab112279)
      12.5% SDS-PAGE Stained with Coomassie Blue with recombinant protein.
    • Western blot
      Western blot
      Anti-DAZL antibody (ab34139) at 1 µg/ml + DAZL protein (ab112279) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for DAZL protein (ab112279)

    ab112279 has not yet been referenced specifically in any publications.

    Publishing research using ab112279 ? Please let us know so that we can cite the reference in this datasheet

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