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Western blot - DBC1 antibody (ab70239)
(enlarge)
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Product Name
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DBC1 antibody
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See all DBC1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DBC1
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Immunogen
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Synthetic peptide corresponding to a region between residues 1 and 50 of Human DBC1 (SwissProt Q8N163).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 1-10µg/mg of lysate.
WB: 1/10000 - 1/20000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Nuclear extract from HeLa cells
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Estrogen
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
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Relevance
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The DBC1 (Deleted in breast cancer 1) amino terminus binds directly to the estrogen receptor alpha (ERalpha) hormone-binding domain in a strict ligand-independent manner. It may function in the modulation of ERalpha expression and hormone-independent breast cancer cell survival. It is ubiquitously expressed in normal tissues.
DBC1 promotes p53-mediated apoptosis through specific inhibition of SIRT1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 8% Phosphate, PBS, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DBC1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DBC1 antibody images, references, products related to ab70239 and other tools.
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DBC1 antibody images:
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 Western blot - DBC1 antibody (ab70239)
All lanes : DBC1 antibody (ab70239) at 1/10000 dilution
Lane 1 : HeLa cell nuclear extract at 15 µg
Lane 2 : HeLa cell nuclear extract at 45 µg
Predicted band size : 103 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to DBC1
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DBC1 antibody - more information
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Customer reviews (feedback) regarding DBC1 antibody |
Customer FAQs regarding DBC1 antibody |
Protocols for DBC1 antibody |
Price and availability of products related to DBC1 antibody |
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DBC1 antibody - related products:
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DBC1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DBC1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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