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Read our guarantee »Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
Anti-DBC1 antibody
See all DBC1 products (6) ...
Rabbit polyclonal to DBC1
WB, IPmore details
Reacts with
Human
Predicted to work with
Mouse
Synthetic peptide corresponding to a region between residues 1 and 50 of Human DBC1 (SwissProt Q8N163).
Nuclear extract from HeLa cells
Liquid
Store at +4°C.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
Polyclonal
IgG
Western blot - DBC1 antibody (ab70239)
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Our Abpromise guarantee covers the use of ab70239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/10000 - 1/20000.Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
IP: Use at 1-10 µg/mg of lysate.
The DBC1 (Deleted in breast cancer 1) amino terminus binds directly to the estrogen receptor alpha (ERalpha) hormone-binding domain in a strict ligand-independent manner. It may function in the modulation of ERalpha expression and hormone-independent breast cancer cell survival. It is ubiquitously expressed in normal tissues. DBC1 promotes p53-mediated apoptosis through specific inhibition of SIRT1.
Cytoplasmic
Western blot - DBC1 antibody (ab70239)

All lanes : Anti-DBC1 antibody (ab70239) at 1/10000 dilution
Lane 1 : HeLa cell nuclear extract at 15 µg
Lane 2 : HeLa cell nuclear extract at 45 µg
Predicted band size : 103 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
ab70239 has not yet been referenced specifically in any publications.
Publishing research using ab70239? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-DBC1 antibody (ab70239) at 1/10000 dilution
Lane 1 : HeLa cell nuclear extract at 15 µg
Lane 2 : HeLa cell nuclear extract at 45 µg
Predicted band size : 103 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
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