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Western blot - DBC1 antibody (ab70240)
(enlarge)
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Product Name
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DBC1 antibody
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See all DBC1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DBC1
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Immunogen
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Synthetic peptide corresponding to a region between residues 650 and 700 of human DBC1 (TrEMBL Q6P0Q9)
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Reacts with
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Hu
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Predicted to react
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 1-10µg/mg of lysate.
WB: 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Nuclear extract from HeLa cells.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Estrogen
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
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Relevance
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The DBC1 (Deleted in breast cancer 1) amino terminus binds directly to the estrogen receptor alpha (ERalpha) hormone-binding domain in a strict ligand-independent manner. It may function in the modulation of ERalpha expression and hormone-independent breast cancer cell survival. It is ubiquitously expressed in normal tissues.
DBC1 promotes p53-mediated apoptosis through specific inhibition of SIRT1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Tris-citrate/phosphate buffer, pH 7 to 8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DBC1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DBC1 antibody images, references, products related to ab70240 and other tools.
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DBC1 antibody images:
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 Western blot - DBC1 antibody (ab70240)
Lanes 1 - 2 : DBC1 antibody (ab70240) at 1/1000 dilution
Lanes 3 - 4 : DBC1 antibody (ab70240) at 1/10000 dilution
Lane 1 : Nuclear extract from HeLa cells at 15 µg
Lane 2 : Nuclear extract from HeLa cells at 45 µg
Lane 3 : Nuclear extract from HeLa cells at 15 µg
Lane 4 : Nuclear extract from HeLa cells at 45 µg
developed using the ECL technique
Predicted band size : 103 kDa
Exposure time : 5 seconds
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Search PubMed (MEDLINE) for references to DBC1
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DBC1 antibody - more information
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Customer reviews (feedback) regarding DBC1 antibody |
Customer FAQs regarding DBC1 antibody |
Protocols for DBC1 antibody |
Price and availability of products related to DBC1 antibody |
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DBC1 antibody - related products:
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DBC1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DBC1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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