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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DBC1 antibody (ab84384)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DBC1 antibody (ab84384)
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Product Name
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DBC1 antibody
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See all DBC1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DBC1
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Immunogen
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Synthetic peptide corresponding to a region between residues 1 and 50 of Human DBC1 (TrEMBL entry Q6P0Q9)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/100 - 1/500. Epitope exposure with citrate buffer will enhance staining.
Note: expected to work on frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human: breast carcinoma, colon carcinoma, metastatic lymph node, ovarian carcinoma, pancreatic islet cell tumor, skin basal cell carcinoma, stomach adenocarcinoma, testicular seminoma.
Mouse: squamous cell carcinoma.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Estrogen
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Estrogen
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Relevance
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The DBC1 (Deleted in breast cancer 1) amino terminus binds directly to the estrogen receptor alpha (ERalpha) hormone-binding domain in a strict ligand-independent manner. It may function in the modulation of ERalpha expression and hormone-independent breast cancer cell survival. It is ubiquitously expressed in normal tissues.
DBC1 promotes p53-mediated apoptosis through specific inhibition of SIRT1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DBC1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DBC1 antibody images, references, products related to ab84384 and other tools.
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DBC1 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DBC1 antibody (ab84384)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast tumor tissue using 1/250 ab84384, followed by DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DBC1 antibody (ab84384)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma tissue using 1/250 ab84384, followed by DAB staining.
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References for DBC1 antibody (ab84384)
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This product has been used in:
Cha EJ et al. Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma. Clin Cancer Res 15:4453-9 (2009). PubMed: 19509139
If you publish research using ab84384 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to DBC1
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DBC1 antibody - more information
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Customer reviews (feedback) regarding DBC1 antibody |
Customer FAQs regarding DBC1 antibody |
Protocols for DBC1 antibody |
Price and availability of products related to DBC1 antibody |
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DBC1 antibody - related products:
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DBC1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DBC1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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