![Western blot - BDF4 antibody [2047C4a] (ab50624) image](#ab50624.gif)
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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-DBF4 antibody [2047C4a]
See all DBF4 products (5) ...
Description
Mouse monoclonal [2047C4a] to DBF4
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human DBF4
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate) pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab50624 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
Clonality
Monoclonal
Clone number
2047C4a
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
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Western blot - BDF4 antibody [2047C4a] (ab50624)
![Western blot - BDF4 antibody [2047C4a] (ab50624) image](/ps/datasheet/Images/50/ab50624/ab50624.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 36 kDa (predicted molecular weight: 77 kDa).
Dot: Use at an assay dependent dilution.
Target
Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Tissue specificity
Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Sequence similarities
Contains 2 BRCT domains.
Contains 1 DBF4-type zinc finger.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UBU7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator of S phase kinase antibody
- ASK antibody
- Chiffon homolog A antibody
see all
Database links
- Entrez Gene: 10926 Human
- Omim: 604281 Human
- SwissProt: Q9UBU7 Human
- Unigene: 485380 Human
Anti-DBF4 antibody [2047C4a] images:
Western blot - BDF4 antibody [2047C4a] (ab50624)
Anti-DBF4 antibody [2047C4a] (ab50624) + immunized recombinant protein
Predicted band size : 77 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
References for Anti-DBF4 antibody [2047C4a] (ab50624)
ab50624 has not yet been referenced specifically in any publications.
Publishing research using ab50624? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"