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Western blot - DBF4 antibody (ab70134)
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Immunocytochemistry/ Immunofluorescence-DBF4 antibody(ab70134)
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Product Name
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DBF4 antibody
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See all DBF4 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DBF4
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Immunogen
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Synthetic peptide derived from the N terminus of human DBF4.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab70134 detects endogenous levels of total DBF4 protein.
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Tested applications
(see key)
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH3T3 cell extracts treated with H2O2 (100µM, 30mins), COS7 cell extracts treated with PMA (125mg/ml, 30mins), and NIH3T3 cell extracts treated with both H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins).
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
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Relevance
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DBF4 plays a role as a regulatory subunit for CDC7 which activates its kinase activity. Is required for entry into S phase of the cell cycle. The complex CDC7-DBF4 selectively phosphorylates MCM2 subunit of chromatin-associated MCM complex and then is involved in regulating the initiation of DNA replication during cell cycle.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab70124 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DBF4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DBF4 antibody images, references, products related to ab70134 and other tools.
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DBF4 antibody images:
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 Western blot - DBF4 antibody (ab70134)
All lanes : DBF4 antibody (ab70134) at 1/500 dilution
Lane 1 : NIH3T3 cell extracts
treated with H2O2 (100µM, 30mins) at 5 µg
Lane 2 : COS7 cell extracts
treated with PMA (125mg/ml, 30mins)
Lane 3 : NIH3T3 cell extracts
treated with H2O2 (100µM, 30mins) and PMA (125mg/ml, 30mins) with immunising peptide at 5 µg
Predicted band size : 77 kDa
Observed band size : 77 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence-DBF4 antibody(ab70134)
ICC/IF image of ab70134 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to DBF4
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DBF4 antibody - more information
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Customer reviews (feedback) regarding DBF4 antibody |
Customer FAQs regarding DBF4 antibody |
Protocols for DBF4 antibody |
Price and availability of products related to DBF4 antibody |
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DBF4 antibody - related products:
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DBF4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about DBF4 antibody |
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