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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-DBF4B antibody
See all DBF4B products (2) ...
Description
Mouse polyclonal to DBF4B
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length human DBF4B protein (NP_079380.1)
Positive control
DBF4B transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Western blot - DBF4B antibody (ab69154)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: Isoform 4, 18 kDa; Isoform 1, 67 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.
Tissue specificity
Widely expressed. Highly expressed in testis.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 DBF4-type zinc finger.
Developmental stage
Increases as cells enter in S phase through G2/M phase. The protein has a short half-life (at protein level).
Post-translational
modifications
Phosphorylated.
Cellular localization
Nucleus. Predominantly found in soluble fraction but not in the chromatin-bound fraction.
Target information above from: UniProt accessionQ8NFT6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator of S phase kinase like protein 1 antibody
- Activator of S phase kinase-like protein 1 antibody
- ASK like protein 1 antibody
see all
Database links
- Entrez Gene: 80174 Human
- Omim: 611661 Human
- SwissProt: Q8NFT6 Human
- Unigene: 369998 Human
- Unigene: 728986 Human
Anti-DBF4B antibody images:
Western blot - DBF4B antibody (ab69154)
All lanes : Anti-DBF4B antibody (ab69154) at 1/500 dilution
Lane 1 : DBF4B transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 18 kDa
Observed band size : 18 kDa
References for Anti-DBF4B antibody (ab69154)
ab69154 has not yet been referenced specifically in any publications.
Publishing research using ab69154? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"