Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Overview
Product name
Anti-DBT antibody
See all DBT products (3) ...
Description
Mouse polyclonal to DBT
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human) (AAH16675.1)
Positive control
Transfected 293T cell line Human liver tissue lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Amino Acids
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 53 kDa.
Target
Relevance
DBT or dihydrolipoamide branched chain transacylase E2 is a component of the BCKD (branched-chain alpha-keto dehydrogenase) complex which is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. The BCKD complex is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine.
Cellular localization
Mitochondrion matrix.
Alternative names
- E2B antibody
- BCATE2 antibody
- BCKAD E2 subunit antibody
see all
Database links
- Entrez Gene: 1629 Human
- Omim: 248610 Human
- SwissProt: P11182 Human
Anti-DBT antibody images:
Western blot - DBT antibody (ab88471)
Anti-DBT antibody (ab88471) at 1 µg/ml + Human liver tissue lysate at 50 µg
Secondary
Goat Anti-Mouse IgG at 1/5000 dilution
Predicted band size : 53 kDa
Western blot - DBT antibody (ab88471)
All lanes : Anti-DBT antibody (ab88471) at 1 µg/ml
Lane 1 : DBT transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG at 1/5000 dilution
Lane 2 : Goat Anti-Mouse IgG at 1/5000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
References for Anti-DBT antibody (ab88471)
ab88471 has not yet been referenced specifically in any publications.
Publishing research using ab88471? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"