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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-DBX1 antibody
See all DBX1 products (2) ...
Description
Rabbit polyclonal to DBX1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (GCREQTLPTK LNPHPDLSDV GQKGPGNEEE EEGPGSPSHR LAYHASSDPQ) of Human DBX1 (NP_001025036).
GCREQTLPTK LNPHPDLSDV GQKGPGNEEE EEGPGSPSHR LAYHASSDPQ
Positive control
Human fetal spleen lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Neural Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> HOX
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Western blot - DBX1 antibody (ab87327)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Target
Relevance
Dbx1 homeodomain transcription factor is expressed in progenitors at the boundary between the dorsal and ventral plates of the caudal neural tube, from which postmitotic cells migrate tangentially to their final destination. Dbx1 is implicated in patterning the central nervous system during embryogenesis. Cell fate allocation and cell diversity are determined at very early stages in progenitor cells at precise coordinates along the dorsoventral and anteroposterior axis. In the spinal cord, the spatially restricted expression of Dbx1 in progenitors is critical in establishing interneuron cell fates and helps coordinate diverse phenotypic features. In the telencephalon, Dbx1 is expressed in restricted progenitor domains at the borders of the developing pallium.
Cellular localization
Nuclear
Alternative names
- dbx antibody
- Developing brain homeobox 1 antibody
- Developing brain homeobox protein 1 antibody
- Homeobox protein DBX1 antibody
Database links
- Entrez Gene: 120237 Human
- Entrez Gene: 13172 Mouse
- Entrez Gene: 292934 Rat
- SwissProt: A6NMT0 Human
- SwissProt: P52950 Mouse
- SwissProt: Q5NSW5 Rat
- Unigene: 104881 Rat
Anti-DBX1 antibody images:
Western blot - DBX1 antibody (ab87327)
Anti-DBX1 antibody (ab87327) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 55 kDa,<22 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
References for Anti-DBX1 antibody (ab87327)
ab87327 has not yet been referenced specifically in any publications.
Publishing research using ab87327? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"