DC-SIGN antibody [120507] (ab13487)

Product Name 

DC-SIGN antibody [120507]

Product type 

Primary antibodies

Description 

Mouse monoclonal [120507] to DC-SIGN

Immunogen 

Tissue / cell preparation (NIH/3T3 transfectants stably expressing human DC-SIGN1).

Reacts with 
(species key)

Hu

Specificity 

The antibody does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

Tested applications 
(see key)

Flow Cyt, Inhib

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at a concentration of 25 - 50 µg/ml (10 µl of a 25-50 µg/ml stock solution of the monoclonal antibody is mixed with up to 1 million cells in a minimal volume (< 0.2 ml) of buffer (PBS + 0.5% BSA). The reaction is incubated at room temperature for 30 minutes.
Inhib: Use at a concentration of 5 µg/ml. (This antibody shows inhibition of DC-SIGN expressing NIH-3T3 cell adhesion to ICAM-3 coated 96 well plates. The adhesion of NIH-3T3-DC-SIGN cells (5 x 104 cells/well) to plates coated with 5 µg/ml (100 µl/well) of recombinant human ICAM-3/Fc is maximally inhibited (100%) using 100 µl of a 5 µg/ml antibody solution per well.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Transfected NIH/3T3 cells and monocyte derived dendritic cells.

Cellular localization 

Cell membrane; Single-pass type II membrane protein (isoform 1 to isoform 5) and Secreted (isoform 6 to isoform 13)

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> T Cell Adhesion
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Adaptive Immunity >> T Cells >> CD

Relevance 

Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV1, HIV2 and SIV bind to DCs via DCSIGN. ICAM3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed CD299, DC-SIGNR, L-SIGN and DCSIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

120507

Isotype 

IgG2b

Purity 

Protein G purified

Storage buffer 

Preservative: 0.08% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for DC-SIGN antibody [120507] (ab13487)

  This product has been used in: (two most recent references)

Zenaro E et al. Induction of Th1/Th17 immune response by Mycobacterium tuberculosis: role of dectin-1, Mannose Receptor, and DC-SIGN. J Leukoc Biol 86:1393-401 (2009). PubMed: 19773555

Brown MG et al. Dramatic caspase-dependent apoptosis in antibody-enhanced dengue virus infection of human mast cells. J Leukoc Biol : (2008). Flow Cyt; Human. PubMed: 18809735

See all 3 publication references for this product.


If you publish research using ab13487 please let us know so that we can cite the reference on this datasheet.

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