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ab110799 |
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ab37994 has been referenced in 6 publications.
Publishing research using ab37994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-DCAMKL1 antibody (ab37994) + mouse heart tissue lysate
Predicted band size : 82 kDa
Observed band size : 82 kDa
Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
Ab37994 at (conc) staining human hepatocarcinoma; formalin fixed, paraffin embedded. Ab37994 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Anti-DCAMKL1 antibody (ab37994) at 1/750 dilution (for 20 hours at 4°C) + Human brain whole tissue lysate at 22 µg
Secondary
An undiluted HRP-conjugated Goat anti-rabbit polyclonal
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 82 kDa
Exposure time : 3 minutes
Blocking step: 5% Milk for 1 hour at 18C°C
This image is courtesy of an anonymous Abreview
ICC/IF image of ab37994 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37994, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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