|
|
|
|
|
Product Name
|
DCC antibody [DM51]
|
| |
See all DCC antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [DM51] to DCC
|
|
Immunogen
|
Prokaryotic recombinant fusion protein corresponding to a 205 amino acid portion of the intracellular domain of the Human DCC molecule.
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IHC-P
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: 1/20 - 1/40.
High temperature antigen unmasking technique. 60 minutes primary antibody incubation at 25°C recommended.
Is unsuitable for IHC-Fr or WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human bowel tissue.
|
|
Cellular localization
|
Membrane; single pass type I membrane protein.
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Cell Biology >> Apoptosis >> Receptors >> Associated Proteins
|
|
Relevance
|
Deleted in colorectal cancer (DCC, chromosome 18q21) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. The gene has also been deleted in several other types of cancer. The DCC protein is a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. The extracellular domain is composed of four Ig like domains and six fibronectin type III repeats. Native DCC is found in three isoforms. Two forms, a long and a short isoform, are produced from the same gene but have different initiation sites. The third isoform, produced by alternative splicing, is expressed only in embryonic tissue. Mouse DCC extracellular domain shares 97% and 99% homology with human and rat DCC extracellular domains, respectively. In adults, DCC is highly expressed in the brain but is also expressed at very low levels in multiple normal tissues. In the embyro, high levels of expression are detected in the brain and neural tube. DCC functions as a receptor or a component of a receptor for netrins and mediates the effects of netrins on commissural axons. Netrins are chemoattractants responsible for the guidance of commissural axons at the midline and of motor axons to the their target muscles. DCC induces apoptosis in the absence of ligand binding, blocks apoptosis when engaged by netrin 1, and also acts as a caspase substrate.
|
|
|
DCC antibody [DM51] (ab49169) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
DM51
|
|
Myeloma
|
P3-NS1/1-Ag4-1
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
Tissue culture supernatant
|
|
Storage buffer
|
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DCC antibody [DM51] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to DCC
|
| |
DCC antibody [DM51] - more information
|
Customer reviews (feedback) regarding DCC antibody [DM51] |
Customer FAQs regarding DCC antibody [DM51] |
Protocols for DCC antibody [DM51] |
Price and availability of products related to DCC antibody [DM51] |
| |
DCC antibody [DM51] - related products:
|
|
|
| |
DCC antibody [DM51] - other tools:
|
Contact Abcam with a Technical Enquiry about DCC antibody [DM51] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
